Anti-PAPLN/Papilin 抗体 [5B2E5] (ab181788)
![Western blot - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)](https://www.abcam.co.jp/ps/products/181/ab181788/Images/ab181788-211055-ab181788WB1.jpg "Western blot - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)")
Key features and details
- Mouse monoclonal [5B2E5] to PAPLN/Papilin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PAPLN/Papilin antibody [5B2E5] -
製品の詳細
Mouse monoclonal [5B2E5] to PAPLN/Papilin -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human PAPLN/Papilin aa 766-870. Expressed in E. Coli.
Sequence:WAARWYFVASVGQCNRFWYGGCHGNANNFASEQECMSSCQGSLHGPRRPQ PGASGRSTHTDGGGSSPAGEQEPSQHRTGAAVQRKPWPSGGLWRQDQQPG PGEAP
Database link: O95428 -
ポジティブ･コントロール
- Human PAPLN/Papilin recombinant protein; HEK293 cell lysate, transfected with PAPLN/Papilin (amino acids 766-870)-IgGFc; HeLa, HepG2, OCM-1, Raji, Jurkat and mouse NIH 3T3 cell lysates; Mouse NIH 3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
5B2E5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181788の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/200 - 1/1000.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 138 kDa.
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| 特記事項 |
|---|
|
ICC/IF
1/200 - 1/1000. |
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WB
1/500 - 1/2000. Predicted molecular weight: 138 kDa. |
ターゲット情報
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配列類似性
Belongs to the papilin family.
Contains 1 BPTI/Kunitz inhibitor domain.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 PLAC domain.
Contains 5 TSP type-1 domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 89932 Human
- Entrez Gene: 170721 Mouse
- SwissProt: O95428 Human
- SwissProt: Q9EPX2 Mouse
- Unigene: 509909 Human
- Unigene: 133443 Mouse
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別名
- Papilin antibody
- papilin, proteoglycan-like sulfated glycoprotein antibody
- PAPLN antibody
see all
画像
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Western blot - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)Anti-PAPLN/Papilin antibody [5B2E5] (ab181788) at 1/500 dilution + Human PAPLN/Papilin recombinant protein
Predicted band size: 138 kDa -
![Western blot - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)](https://www.abcam.co.jp/ps/products/181/ab181788/Images/ab181788-211054-ab181788WB2.jpg)
Western blot - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)All lanes : Anti-PAPLN/Papilin antibody [5B2E5] (ab181788) at 1/500 dilution
Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, transfected with PAPLN/Papilin (amino acids 766-870)-hIgGFc
Predicted band size: 138 kDa -
![Western blot - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)](https://www.abcam.co.jp/ps/products/181/ab181788/Images/ab181788-211053-ab181788WB3.jpg)
Western blot - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)All lanes : Anti-PAPLN/Papilin antibody [5B2E5] (ab181788) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : OCM-1 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : mouse NIH 3T3 cell lysate
Predicted band size: 138 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)](https://www.abcam.co.jp/ps/products/181/ab181788/Images/ab181788-211052-ab181788ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PAPLN/Papilin antibody [5B2E5] (ab181788)Immunofluorescent analysis of mouse NIH 3T3 cells labeling PAPLN/Papilin with ab181788 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab181788 は論文での使用が確認できていません。