Anti-Pancreatic Polypeptide 抗体 (ab113694)
Key features and details
- Rabbit polyclonal to Pancreatic Polypeptide
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Pancreatic Polypeptide antibody
Pancreatic Polypeptide 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Pancreatic Polypeptide -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Human pancreas tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113694の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 100064909 Horse
- Entrez Gene: 5539 Human
- Entrez Gene: 713988 Macaque monkey
- Omim: 167780 Human
- SwissProt: P68010 Horse
- SwissProt: P01298 Human
- SwissProt: P33684 Macaque monkey
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別名
- Pancreatic polypeptide antibody
- Pancreatic polypeptide Y antibody
- Pancreatic prohormone antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody (ab113694)IHC image of Pancreatic Polypeptide staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113694, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab113694 は 8 報の論文で使用されています。
- Boehmer BH et al. Chronic Fetal Leucine Infusion Does Not Potentiate Glucose-Stimulated Insulin Secretion or Affect Pancreatic Islet Development in Late-Gestation Growth-Restricted Fetal Sheep. J Nutr 151:312-319 (2021). PubMed: 33326574
- Yoshihara E et al. Immune-evasive human islet-like organoids ameliorate diabetes. Nature 586:606-611 (2020). PubMed: 32814902
- Boehmer BH et al. A Chronic Fetal Leucine Infusion Potentiates Fetal Insulin Secretion and Increases Pancreatic Islet Size, Vascularity, and ß Cells in Late-Gestation Sheep. J Nutr 150:2061-2069 (2020). PubMed: 32470982
- Menegaz D et al. Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell. Nat Metab 1:1110-1126 (2019). PubMed: 32432213
- Katoh MC et al. MafB Is Critical for Glucagon Production and Secretion in Mouse Pancreatic a Cells In Vivo. Mol Cell Biol 38:N/A (2018). PubMed: 29378833
- Moin ASM et al. Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas. Front Endocrinol (Lausanne) 9:791 (2018). PubMed: 30687234
- Phelps EA et al. Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation. Sci Rep 7:45961 (2017). PubMed: 28401888
- Xiao X et al. Transient Suppression of TGFß Receptor Signaling Facilitates Human Islet Transplantation. Endocrinology 157:1348-56 (2016). PubMed: 26872091