Anti-Pan Trk 抗体 [EPR24009-335] - BSA and Azide free (ab284409)
![Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486163-24009WB1.jpg "Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24009-335] to Pan Trk - BSA and Azide free
- Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free
Pan Trk 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24009-335] to Pan Trk - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IP, Flow Cyt (Intra)more details
適用なし: ICC/IF or IHC-Fr -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Whole cell lysates: 293T (human embryonic kidney) transfected with vectors containing a myc-His-tag® and: control, TrkB, TrkC, TrcA. Human, mouse and rat brain tissue lysates. IHC-P: Human glioblastoma and cerebrum, mouse and rat cerebrum, HEK-293T transfected with TrkC or TrkA or TrkB expression vector. Flow cyt. Intr.: ICR Mouse primary neuron cells, SD Rat primary neuron. IP: Mouse and rat brain lysates.
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特記事項
ab284409 is a carrier free version of ab267830.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24009-335 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab284409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 92 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 92 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane. -
参照データベース
- Entrez Gene: 4914 Human
- Entrez Gene: 4915 Human
- Entrez Gene: 4916 Human
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 18213 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 29613 Rat
see all -
別名
- BDNF/NT-3 growth factors receptor antibody
- EML4 antibody
- ETV6 antibody
see all
画像
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Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)All lanes : Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
Lane 2 : 293T transfected with human TrkB expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : 293T transfected with human TrkC expression vector containing a myc-His-tag®, whole cell lysate
Lane 4 : 293T transfected with human TrkA expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab267830, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486173-24009WB2.jpg)
Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)All lanes : Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : Human kidney tissue lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 1/20 dilution per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 140, 32 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using ab267830, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The 32KDa band may be an intracellular fragment of TrkB (TrkB-ICD). The observed Mw of ~140KDa is higher than the predicted Mw due to glycosylation (PMID:24860020).
Negative control: heart, kidney, spleen (PMID: 7823156). -
![Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486174-24009WB3.jpg)
Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)All lanes : Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140, 32 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsThis data was developed using ab267830, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The 32KDa band may be an intracellular fragment of TrkB (TrkB-ICD). The observed Mw of ~140KDa is higher than the predicted Mw due to glycosylation (PMID:24860020).
Negative control: heart, kidney, spleen (PMID: 7823156). -
![Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486176-24009WB4.jpg)
Western blot - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)All lanes : Anti-Pan Trk antibody [EPR24009-335] (ab267830) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 1/20 dilution per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 140, 32 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsThis data was developed using ab267830, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The 32KDa band may be an intracellular fragment of TrkB (TrkB-ICD). The observed Mw of ~140KDa is higher than the predicted Mw due to glycosylation (PMID:24860020).
Negative control: heart, kidney, spleen (PMID: 7823156). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486264-IHC1hucerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of human cerebrum labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum (PMID: 26171003).
The section was incubated with ab267830 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486323-IHC2huglioblastoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of human glioblastoma labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human glioblastoma (PMID: 26171003). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab267830 for 30 mins at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486324-IHC3mscerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of mouse cerebrum labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab267830 for 30 mins at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486326-IHC4rtcerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of rat cerebrum labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab267830 for 30 mins at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486328-293TOE89293TOE90293TOE91293TOE200IHC5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections detailed below and labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Sections included:
Panel A: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with TrkC expression vector containing a myc-His-tag®.
Panel B: HEK-293T transfected with TrkA vector containing a myc-His-tag®.
Panel C: HEK-293T transfected with TrkB vector containing a myc-His-tag®.
Panel D: HEK-293T transfected with empty vector containing a myc-His-tag®.Positive staining on (A) HEK-293T transfected with TrkC expression vector, (B) HEK-293T transfected with a TrkA expression vector, and (C) HEK-293T transfected with TrkB expression vector. Almost no staining on (D) HEK-293T transfected with empty vector.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486330-IHC6huliver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of human liver labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on human liver. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab267830 for 30 mins at room temperature. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486332-IHC7hukidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of human kidney labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on human kidney The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab267830 for 30 mins at room temperature. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486334-IHC8-msliver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of mouse liver labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on mouse liver. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab267830 for 30 mins at room temperature. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486337-IHC9rtliver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of rat liver labeling Trk with ab267830 at 1/200 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on rat liver. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab267830 for 30 mins at room temperature. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
-
![Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486339-24009SSCmouse-FCIn.jpg)
Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol permeabilized ICR Mouse primary neuron cells labelling Trk with ab267830 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486341-24009SSCrat-FCint.jpg)
Flow Cytometry (Intracellular) - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed and 90% methanol permeabilized SD Rat primary neuron cells labelling Trk with ab267830 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150097) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486343-24009IP1.jpg)
Immunoprecipitation - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Trk was immunoprecipitated from mouse brain tissue lysate 10 µg with ab267830 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab267830 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 μg (Inset)
Lane 2: ab267830 IP in mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267830 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.
Observed molecular weight [kDa]: 140, 32.
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![Immunoprecipitation - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486345-24009IP2.jpg)
Immunoprecipitation - Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)This data was developed using ab267830, the same antibody clone in a different buffer formulation.
Trk was immunoprecipitated from rat brain tissue lysate 10 µg with ab267830 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab267830 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate 10 μg (Inset)
Lane 2: ab267830 IP in rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267830 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.
Observed molecular weight [kDa]: 140, 32.
-
![Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)](https://www.abcam.co.jp/ps/products/284/ab284409/Images/ab284409-486161-Benefits-of-Recombinant-antibodies.jpg)
Anti-Pan Trk antibody [EPR24009-335] - BSA and Azide free (ab284409)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab284409 は論文での使用が確認できていません。
