Anti-Pan Trk 抗体 [EPR17341] (ab181560)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17341] to Pan Trk
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Pan Trk antibody [EPR17341]
Pan Trk 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17341] to Pan Trk -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal brain and cerebellum lysates, Mouse and Rat brain lysates. IHC-P: Human astrocytoma and cerebral cortex tissue, Mouse cerebral cortex tissue, Rat cerebral cortex tissue. ICC/IF: Neuro-2a cells.
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特記事項
This is the Research Use Only (RUO) antibody of the clone that has been used in the in vitro diagnostic VENTANA pan-TRK (EPR17341) assay (an immunohistochemistry assay).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17341 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181560の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/250.
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IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
1/10000. Detects a band of approximately 30,140 kDa (predicted molecular weight: 92 kDa).
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特記事項 |
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ICC/IF
1/250. |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/10000. Detects a band of approximately 30,140 kDa (predicted molecular weight: 92 kDa). |
ターゲット情報
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関連性
Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane. -
参照データベース
- Entrez Gene: 4914 Human
- Entrez Gene: 4915 Human
- Entrez Gene: 4916 Human
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 18213 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 29613 Rat
see all -
別名
- BDNF/NT-3 growth factors receptor antibody
- EML4 antibody
- ETV6 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Immunohistochemical analysis of formalin fixed paraffin embedded human cerebrum labelling Pan Trk with ab181560 at a concentration of 5µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. ab181560 anti-Pan Trk antibody [EPR17341] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Immunohistochemical analysis of formalin fixed paraffin embedded human cerebrum labelling Pan Trk with ab181560 at a concentration of 3µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab181560 anti-Pan Trk antibody [EPR17341] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural mix culture cells labelling Pan Trk with ab181560 at 1:100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1:1000 dilution (Green). Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1:200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling Pan Trk with ab181560 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). Confocal image showing cytoplasmic staining on Neuro-2a cells is shown. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (Tubulin mouse mAb) at 1/500 and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab181560 at 1/250 dilution followed by ab150120 (Goat anti mouse IgG (Alexa Fluor® 594)) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Goat anti rabbit IgG (Alexa Fluor® 488)) at 1/400 dilution. -
All lanes : Extraction Buffer (ab191560) at 1/10000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat kidney lysates
Lane 6 : Rat spleen lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 92 kDaTrkB is abundantly expressed in the central and peripheral nervous system. Mouse kidney, mouse spleen, rat kidney and rat spleen are used as negative control.
The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Astrocytoma cells show strong cytoplasmic staining. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
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All lanes : Anti-Pan Trk antibody [EPR17341] (ab181560) at 1/10000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human fetal spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 92 kDaTrkB is abundantly expressed in the central and peripheral nervous systems,
human fetal heart and human fetal spleen are used as negative controls.
The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
Blocking/dilution buffer: 5% NFDM/TBST.
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Different batches of ab181560 were tested on human brain lysate at 1.5 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 30,140 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of human cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
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Anti-Pan Trk antibody [EPR17341] (ab181560) at 1/10000 dilution + Human cerebellum lysates at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 92 kDaTrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.
Blocking/dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of mouse cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining is observed on neurons of Rat cerebral cortex. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Immunohistochemical analysis of paraffin-embedded Human (Panel 1), Mouse (Panel 2) or Rat (Panel 3) liver tissue labeling Pan Trk with ab181560 at 1/500 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. The staining is negative on normal Human liver. Counter stained with Hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Tissue Microarrays stained for "Anti-Pan Trk antibody [EPR17341]” using "ab181560" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab181560 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pan Trk antibody [EPR17341] (ab181560)
Tissue Microarrays stained for "Anti-Pan Trk antibody [EPR17341]” using "ab181560" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab181560 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (37)
ab181560 は 37 報の論文で使用されています。
- Mohamed F et al. The Diagnostic Value of Pan-Trk Expression to Detect Neurotrophic Tyrosine Receptor Kinase (NTRK) Gene Fusion in CNS Tumours: A Study Using Next-Generation Sequencing Platform. Pathol Oncol Res 28:1610233 (2022). PubMed: 35295612
- Ge Y et al. Acute myocardial infarction induces remodeling of the murine superior cervical ganglia and the carotid body. Front Cardiovasc Med 9:758265 (2022). PubMed: 36277772
- Chen Y et al. Case Report: A novel LHFPL3::NTRK2 fusion in dysembryoplastic neuroepithelial tumor. Front Oncol 12:1064817 (2022). PubMed: 36531047
- Garrido P et al. Multidisciplinary consensus on optimising the detection of NTRK gene alterations in tumours. Clin Transl Oncol 23:1529-1541 (2021). PubMed: 33620682
- Fu Y et al. MLH1/PMS2 Expression Could Tell Classical NTRK Fusion in Fluorescence In Situ Hybridization Positive Colorectal Carcinomas. Front Oncol 11:669197 (2021). PubMed: 33996597