Anti-pan Cytokeratin 抗体 [AE1+AE3], prediluted (ab961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse recombinant multiclonal [AE1+AE3] to pan Cytokeratin, prediluted
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-pan Cytokeratin antibody [AE1+AE3], prediluted
pan Cytokeratin 一次抗体 製品一覧 -
製品の詳細
Mouse recombinant multiclonal [AE1+AE3] to pan Cytokeratin, prediluted -
由来種
Mouse -
特異性
This antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa. -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish -
免疫原
Tissue, cells or virus corresponding to pan Cytokeratin. Human epidermal cytokeratins
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ポジティブ・コントロール
- Skin or adenocarcinoma.
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特記事項
This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Proprietary Preservative -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
Recombinant Multiclonal -
クローン名
AE1+AE3 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Unmasking reagent
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab961の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (3) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 19852942
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 19852942 |
ターゲット情報
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関連性
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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別名
- pan ck antibody
- pan-ck antibody
- panck antibody
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (42)
ab961 は 42 報の論文で使用されています。
- Yoo MJ et al. Anti-cancer effect of palmatine through inhibition of the PI3K/AKT pathway in canine mammary gland tumor CMT-U27 cells. BMC Vet Res 19:223 (2023). PubMed: 37880653
- Shmerling M et al. LY6S, a New IFN-Inducible Human Member of the Ly6a Subfamily Expressed by Spleen Cells and Associated with Inflammation and Viral Resistance. Immunohorizons 6:253-272 (2022). PubMed: 35440514
- Yang H & Lan L Immunostaining and Protein Pull-Down of Methyl-5-Cytosine-Marked RNA:DNA Hybrids. Methods Mol Biol 2528:271-276 (2022). PubMed: 35704197
- Yao HJ et al. Three new experimental models of anterior urethral stricture in rabbits. Transl Androl Urol 11:761-772 (2022). PubMed: 35812200
- Park JK et al. Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma. Evid Based Complement Alternat Med 2022:6295910 (2022). PubMed: 36110191