Anti-pan Cadherin 抗体

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (AB16505)

HeLa cells fixed in methanol and stained with ab16505 (2μg/ml). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS. The 2 images show the cells stained with different secondary antibodies, Donkey anti Rabbit FITC (image A) and Donkey anti Rabbit Cy3 (image B). In each case ab16505 stains the plasma membrane. In image A ab16505 is stained green and in image B ab16505 is stained red. In both images the DNA is stained with DAPI (blue).

This image is courtesy of Rosmaria Mangiacasale & Patrizia Lavia, University La Sapienza

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody (AB16505)

IHC image of pan Cadherin staining in human liver FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16505, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (AB16505)

ab16505 staining pan Cadherin in U2OS cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16505 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (AB16505)

Immunofluorescence analysis of HeLa cells, staining pan Cadherin (red) with ab16505.

Cells were fixed with paraformaldehyde, permeabilized in methanol and blocked for 1 hour at room temperature in DPBS containing 2 mg/mL BSA, 1% fish gelatin, 0.1% Triton-X 100 and 5% goat serum. Cells were then incubated for 1 hour at room temperature with the primary antibody diluted in blocking buffer. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Image from Kiss K et al., PLoS One. 2012;7(5):e37378. Epub 2012 May 24. Fig 1.; doi:10.1371/journal.pone.0037378; May 24, 2012, PLoS ONE 7(5): e37378.

• WB

Project788****

Western blot - Anti-pan Cadherin antibody (AB16505)

All lanes:

Western blot - Anti-pan Cadherin antibody (ab16505) at 1 µg/mL

Lane 1:

Mouse heart at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

3T3 cell lysate at 20 µg

Lane 4:

Mouse muscle at 20 µg

Lane 5:

Human heart at 20 µg

Lane 6:

Mouse heart at 20 µg with Human pan Cadherin peptide (ab17098)

Lane 7:

HeLa cell lysate at 20 µg with Human pan Cadherin peptide (ab17098)

Lane 8:

3T3 cell lysate at 20 µg with Human pan Cadherin peptide (ab17098)

Lane 9:

Mouse muscle at 20 µg with Human pan Cadherin peptide (ab17098)

Lane 10:

Human heart at 20 µg with Human pan Cadherin peptide (ab17098)

Secondary

All lanes:

Goat anti-rabbit conjugated to Alexafluor 680 at 1/10000 dilution

Predicted band size: 100 kDa

Observed band size: 135 kDa

false

• WB

Project1232****

Western blot - Anti-pan Cadherin antibody (AB16505)

All lanes:

Western blot - Anti-pan Cadherin antibody (ab16505) at 1 µg/mL

Lane 1:

Human heart lysate at 20 µg

Lane 2:

Mouse heart lysate at 20 µg

Lane 3:

Rat heart lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab7090'>ab7090</a>) at 1/5000 dilution

Predicted band size: 100 kDa

Observed band size: 125-140 kDa,40 kDa,65 kDa,75 kDa,90 kDa

true

Exposure time: 1min