AB2871

Anti-pan ATPase Alpha 抗体 [M7-PB-E9]

Name: Anti-pan ATPase Alpha 抗体 [M7-PB-E9] (ab2871) | Abcam
Brand: Abcam Limited
SKU: AB2871
Price: 73000 JPY
Availability: InStock

Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control

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(9 Publications)

Mouse Monoclonal alpha 1 Sodium Potassium ATPase antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 9 publications. Immunogen corresponding to Native Full Length Protein corresponding to Sheep ATP1A1.

別名を表示する

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

8 Images

IHC-P

Unknown

Immunohistochemistry paraffin embedded sections - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human testis tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha ab2871 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

IHC-P

Unknown

Immunohistochemistry paraffin embedded sections - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha ab2871 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunocytochemistry/Immunofluorescence analysis of ATP1A2 shows staining in MCF-7 cells. ATP1A2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2871 (1 : 20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunocytochemistry/Immunofluorescence analysis of ATP1A2 shows staining in U251 cells. ATP1A2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2871 (1 : 20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

ICC/IF image of ab2871 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2871, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC-P

Unknown

Immunohistochemistry paraffin embedded sections - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 100 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha ab2871 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Flow Cyt

Unknown

Flow Cytometry - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Overlay histogram showing HEK293 cells stained with ab2871 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28711, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunocytochemistry/Immunofluorescence analysis of ATP1A2 shows staining in HeLa cells. ATP1A2 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2871 (1 : 20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

M7-PB-E9

アイソタイプ

IgG1

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IP, Flow Cyt, ICC/IF, WB, IHC-P

applications

免疫原

Native Full Length Protein corresponding to Sheep ATP1A1. The exact immunogen used to generate this antibody is proprietary information.

P04074

エピトープ

This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium / potassium ATPase.

特異性

Detects pan alpha sodium / potassium ATPase. The antibody binds all alpha subunits in sheep, dog, pig, human and chicken. It does not bind alpha 1 or 2 in rat, but does bind alpha 3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/200", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.05% Sodium azide Constituents: 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Pan ATPase Alpha also known as 'alpha pan' or 'pb alpha' is a protein complex with an approximate molecular mass of 100 kDa. It is part of a larger family of ATPases which hydrolyze ATP to ADP releasing energy used in various cellular processes. The protein is expressed in diverse tissues in the body reflecting its essential role in cellular metabolism. It consists of multiple subunits that work together to maintain the proton gradient across membranes important for energy homeostasis.

Biological function summary

Different subunits of ATPase Alpha are important for cellular energy metabolism and are part of a larger enzymatic complex. This complex facilitates proton translocation in cellular organelles contributing to energy conversion processes. Through ATP hydrolysis this protein helps maintain ion gradients which are employed by cells for multiple functions such as nutrient uptake and pH balance. The machinery provided by ATPase Alpha is vital for cell survival and function.

Pathways

The activity of ATPase Alpha integrates into significant metabolic pathways such as oxidative phosphorylation and glycolysis. It is central in energy transduction providing ATP required for many cellular activities. The protein modulates activity in conjunction with other components like the cytochrome c oxidase complex in the mitochondrial electron transport chain playing a role in producing the cell's energy currency. Pan ATPase Alpha's ability to interface with glycolysis allows cells to effectively manage energy production and consumption.

Abnormal levels of ATPase Alpha are linked to conditions such as metabolic syndrome and neurodegenerative diseases. In metabolic syndrome dysregulation of ATPase Alpha may contribute to impaired energy metabolism and insulin resistance. Additionally alterations in its function can relate to neurodegenerative disorders where mitochondrial dysfunction is prevalent. In these diseases ATPase Alpha interacts with other key proteins such as amyloid precursor protein in Alzheimer's disease indicating its possible role in disease pathogenesis and progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

See full target information ATP1A1

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Neuropathology and applied neurobiology 48:e12811 PubMed35274343

2022

Extracellular tau oligomers affect extracellular glutamate handling by astrocytes through downregulation of GLT-1 expression and impairment of NKA1A2 function.

Applications

Unspecified application

Species

Unspecified reactive species

Domenica Donatella Li Puma,Cristian Ripoli,Giulia Puliatti,Francesco Pastore,Giacomo Lazzarino,Barbara Tavazzi,Ottavio Arancio,Roberto Piacentini,Claudio Grassi

International journal of molecular sciences 23: PubMed35008753

2021

Berberine Decreases Intestinal GLUT2 Translocation and Reduces Intestinal Glucose Absorption in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhang,Hongyan Yang,Erwan Yang,Jia Li,Ling Dong

International journal of molecular sciences 22: PubMed34066474

2021

Mesenchymal Stem Cell-Derived Extracellular Vesicles Protect Human Corneal Endothelial Cells from Endoplasmic Reticulum Stress-Mediated Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lola Buono,Simona Scalabrin,Marco De Iuliis,Adele Tanzi,Cristina Grange,Marta Tapparo,Raffaele Nuzzi,Benedetta Bussolati

Frontiers in neuroscience 13:831 PubMed31440132

2019

β-Catenin Controls the Electrophysiologic Properties of Skeletal Muscle Cells by Regulating the α2 Isoform of Na/K-ATPase.

Applications

Unspecified application

Species

Unspecified reactive species

Congying Zhao,Yonglin Yu,Yi Zhang,Jue Shen,Lihua Jiang,Guoxia Sheng,Weiqin Zhang,Lu Xu,Kewen Jiang,Shanshan Mao,Peifang Jiang,Feng Gao

British journal of pharmacology 176:787-800 PubMed30592786

2019

Epigenetic events involved in organic cation transporter 1-dependent impaired response of hepatocellular carcinoma to sorafenib.

Applications

Unspecified application

Species

Unspecified reactive species

Ruba Al-Abdulla,Elisa Lozano,Rocio I R Macias,Maria J Monte,Oscar Briz,Colm J O'Rourke,Maria A Serrano,Jesus M Banales,Matias A Avila,Maria L Martinez-Chantar,Andreas Geier,Jesper B Andersen,Jose J G Marin

The Journal of pathology 246:217-230 PubMed29984492

2018

Increased jejunal permeability in human obesity is revealed by a lipid challenge and is linked to inflammation and type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Laurent Genser,Doriane Aguanno,Hédi A Soula,Liping Dong,Laurence Trystram,Karen Assmann,Joe-Elie Salem,Jean-Christophe Vaillant,Jean-Michel Oppert,Fabienne Laugerette,Marie-Caroline Michalski,Philippe Wind,Monique Rousset,Edith Brot-Laroche,Armelle Leturque,Karine Clément,Sophie Thenet,Christine Poitou

Diabetes 60:2598-607 PubMed21852673

2011

GLUT2 accumulation in enterocyte apical and intracellular membranes: a study in morbidly obese human subjects and ob/ob and high fat-fed mice.

Applications

IHC-Fr, WB

Species

Human, Human

Amal Ait-Omar,Milena Monteiro-Sepulveda,Christine Poitou,Maude Le Gall,Aurélie Cotillard,Jules Gilet,Kevin Garbin,Anne Houllier,Danièle Château,Amélie Lacombe,Nicolas Veyrie,Danielle Hugol,Joan Tordjman,Christophe Magnan,Patricia Serradas,Karine Clément,Armelle Leturque,Edith Brot-Laroche

Nano letters 9:4489-93 PubMed19807066

2009

Localization of Na+-K+ ATPases in quasi-native cell membranes.

Applications

Unspecified application

Species

Human

Junguang Jiang,Xian Hao,Mingjun Cai,Yuping Shan,Xin Shang,Zhiyong Tang,Hongda Wang

Biochimica et biophysica acta 719:413-23 PubMed6295504

1982

Isolation and characterization of monoclonal antibodies to (Na+ + K+)-ATPase.

Applications

Unspecified application

Species

Unspecified reactive species

W J Ball,A Schwartz,J L Lessard

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-pan ATPase Alpha 抗体 [M7-PB-E9]

Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control

Immunohistochemistry paraffin embedded sections - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunohistochemistry paraffin embedded sections - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunocytochemistry/ Immunofluorescence - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunocytochemistry/ Immunofluorescence - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunocytochemistry/ Immunofluorescence - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunohistochemistry paraffin embedded sections - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Flow Cytometry - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Immunocytochemistry/ Immunofluorescence - Anti-pan ATPase Alpha antibody [M7-PB-E9] - Plasma Membrane Loading Control (AB2871)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

特異性

組合せ製品

Assay Kits

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (9)

Abcam product promise