Anti-PAK6 (phospho S165) 抗体 (ab41432)
Key features and details
- Rabbit polyclonal to PAK6 (phospho S165)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PAK6 (phospho S165) antibody
PAK6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK6 (phospho S165) -
由来種
Rabbit -
特異性
Phospho-Ser165-specific. The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-Ser165-PAK6 peptide and also by treatment with lambda phosphatase. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human PAK6 (phospho S165).
Database link: Q9NQU5 -
特記事項
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was cross-adsorbed to unphosphorylated-Ser165-PAK6 peptide, then affinity purified using phospho-Ser165-PAK6 peptide (immunogen). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41432の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
ターゲット情報
-
機能
The activated kinase acts on a variety of targets. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated when activated by CDC42/p21. - Information by UniProt
-
参照データベース
- Entrez Gene: 56924 Human
- Entrez Gene: 214230 Mouse
- Entrez Gene: 296078 Rat
- Omim: 608110 Human
- SwissProt: Q9NQU5 Human
- SwissProt: Q3ULB5 Mouse
- Unigene: 513645 Human
- Unigene: 241857 Mouse
-
別名
- CDKN1A activated kinase 6 antibody
- EC 2.7.1.37 antibody
- p21 activated protein kinase 6 antibody
see all
画像
-
Western blot - Anti-PAK6 (phospho S165) antibody (ab41432)Lanes 1-4 : Anti-PAK6 antibody (ab41431) at 1/1000 dilution
Lanes 5-8 : Anti-PAK6 (phospho S165) antibody (ab41432) at 1/1000 dilution
Lanes 1 & 5 : Human PAK6 recombinant protein phosphorylated by ERK2
Lane 2 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
Lane 3 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific peptide
Lane 4 : Human PAK6 recombinant protein phosphorylated by ERK2 with PAK6 N-terminal peptide (ab41431 immunogen)
Lane 6 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
Lane 7 : Human PAK6 recombinant protein phosphorylated by ERK2 with phospho-S165-PAK6 peptide (ab41432 immunogen)
Lane 8 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific phospho-serine peptide
Predicted band size: 75 kDa
Observed band size: 75 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab41432 は論文での使用が確認できていません。