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リコンビナントRabMAb

Anti-PAK4 抗体 [EPR25195-27] - BSA and Azide free (Detector) (ab290665)

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Sandwich ELISA - Anti-PAK4 antibody [EPR25195-27] - BSA and Azide free (Detector) (ab290665)
  • Anti-PAK4 antibody [EPR25195-27] - BSA and Azide free (Detector) (ab290665)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25195-27] to PAK4 - BSA and Azide free (Detector)
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-PAK4 antibody [EPR25195-27] - BSA and Azide free (Detector)
    PAK4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25195-27] to PAK4 - BSA and Azide free (Detector)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab290664 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PAK 4. The recommended pair for sandwich ELISA is:
         Capture: ab290664 , Human PAK 4 Capture Antibody (unconjugated)
         Detector: ab290665 , Human PAK 4 Detector Antibody (unconjugated)
    The reference range value is 0.08 - 10 ng/mL.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290665の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25195-48] to PAK4 - BSA and Azide free (Capture) (ab290664).
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25195-48] to PAK4 - BSA and Azide free (Capture) (ab290664).

ターゲット情報

  • 機能

    Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.

  • 組織特異性

    Highest expression in prostate, testis and colon.

  • 配列類似性

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Autophosphorylated on serine residues when activated by CDC42/p21.
    Phosphorylated on tyrosine residues upon stimulation of FGFR2.
  • Information by UniProt

  • 参照データベース

    • 別名

      • KIAA1142 antibody
      • p21 activated kinase 4 antibody
      • p21 Cdc42/Rac1-actiated kinase 4 antibody
      • P21 protein (Cdc42/Rac) activated kinase 4 antibody
      • p21(CDKN1A) activated kinase 4 antibody
      • p21-activated kinase 4 antibody
      • PAK 4 antibody
      • PAK-4 antibody
      • Pak4 antibody
      • PAK4_HUMAN antibody
      • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody
      • Serine threonine kinase PAK 4 antibody
      • Serine/threonine protein kinase PAK 4 antibody
      • Serine/threonine protein kinase PAK4 antibody
      • Serine/threonine-protein kinase PAK 4 antibody
      see all

    画像

    • Sandwich ELISA - Anti-PAK4 antibody [EPR25195-27] - BSA and Azide free (Detector) (ab290665)
      Sandwich ELISA - Anti-PAK4 antibody [EPR25195-27] - BSA and Azide free (Detector) (ab290665)
      Sandwich ELISA of ab290697 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
    • Anti-PAK4 antibody [EPR25195-27] - BSA and Azide free (Detector) (ab290665)
      Anti-PAK4 antibody [EPR25195-27] - BSA and Azide free (Detector) (ab290665)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290665 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290665 は論文での使用が確認できていません。

    レビューと Q&A

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