Anti-PAK4 抗体 (ab62509)
Key features and details
- Rabbit polyclonal to PAK4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAK4 antibody
PAK4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
A 13 amino acid synthetic peptide from near the center of human PAK4
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ポジティブ･コントロール
- SW480 cell lysate and human colon tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62509の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
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| IHC-P | (1) |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2. -
組織特異性
Highest expression in prostate, testis and colon. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on serine residues when activated by CDC42/p21.
Phosphorylated on tyrosine residues upon stimulation of FGFR2. - Information by UniProt
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参照データベース
- Entrez Gene: 10298 Human
- Omim: 605451 Human
- SwissProt: O96013 Human
- Unigene: 20447 Human
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別名
- KIAA1142 antibody
- p21 activated kinase 4 antibody
- p21 Cdc42/Rac1-actiated kinase 4 antibody
see all
画像
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Western blot - Anti-PAK4 antibody (ab62509)All lanes : Anti-PAK4 antibody (ab62509) at 1 µg/ml
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PAK4 knockout HEK-293T cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-PAK4 antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab62509 was shown to bind specifically to PAK4. A band was observed at 70 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in PAK4 knockout cell line ab266807 (knockout cell lysate ab258560). To generate this image, wild-type and PAK4 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-PAK4 antibody (ab62509)Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/ml
Lane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/ml
All lanes : SW480 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody (ab62509)Immunohistochemistry of PAK4 in paraffin embedded human colon tissue section with ab62509 antibody at 10 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-PAK4 antibody (ab62509)ICC/IF image of ab62509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-PAK4 antibody (ab62509)Immunofluorescence of PAK4 in Human Colon cells using ab62509 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab62509 は 11 報の論文で使用されています。
- Xing S et al. LINC01224 Exhibits Cancer-Promoting Activity in Epithelial Ovarian Cancer Through microRNA-485-5p-Mediated PAK4 Upregulation. Onco Targets Ther 13:5643-5655 (2020). PubMed: 32606778
- Liu D et al. Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis. BMC Cancer 20:107 (2020). PubMed: 32041570
- Chen T et al. PAK4 phosphorylates fumarase and blocks TGF-ß-induced cell growth arrest in lung cancer cells. Cancer Res N/A:N/A (2019). PubMed: 30683654
- Liu Y et al. MicroRNA-663 inhibits the proliferation and invasion of clear cell renal cell carcinoma cells by directly targeting PAK4. Mol Med Rep 19:711-718 (2019). PubMed: 30431118
- Fulciniti M et al. Functional role and therapeutic targeting of p21-activated kinase 4 in multiple myeloma. Blood 129:2233-2245 (2017). PubMed: 28096095