Anti-PAK4 抗体 (ab17974)
Key features and details
- Rabbit polyclonal to PAK4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAK4 antibody
PAK4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Guinea pig, Dog, Pig, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human PAK4 aa 1-100.
Database link: O96013 -
ポジティブ・コントロール
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17974の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 64 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 64 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2. -
組織特異性
Highest expression in prostate, testis and colon. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on serine residues when activated by CDC42/p21.
Phosphorylated on tyrosine residues upon stimulation of FGFR2. - Information by UniProt
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参照データベース
- Entrez Gene: 484513 Dog
- Entrez Gene: 100736193 Guinea pig
- Entrez Gene: 10298 Human
- Entrez Gene: 70584 Mouse
- Entrez Gene: 100627936 Pig
- Entrez Gene: 100341934 Rabbit
- Omim: 605451 Human
- SwissProt: O96013 Human
see all -
別名
- KIAA1142 antibody
- p21 activated kinase 4 antibody
- p21 Cdc42/Rac1-actiated kinase 4 antibody
see all
画像
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All lanes : Anti-PAK4 antibody (ab17974) at 0.04 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 64 kDa
Exposure time: 3 minutes -
PAK4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab17974 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab17974 at 0.4 µg/ml.
Lane 1: ab17974 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab17974 は論文での使用が確認できていません。