Anti-PAK1 (phospho S204) 抗体 (ab79503)
Key features and details
- Rabbit polyclonal to PAK1 (phospho S204)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAK1 (phospho S204) antibody
PAK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK1 (phospho S204) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human PAK1 (phospho S204).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79503の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 61 kDa.
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 61 kDa. |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated when activated by CDC42/p21 and RAC1. -
細胞内局在
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. - Information by UniProt
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参照データベース
- Entrez Gene: 5058 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 29431 Rat
- Omim: 602590 Human
- SwissProt: Q13153 Human
- SwissProt: O88643 Mouse
- SwissProt: P35465 Rat
- Unigene: 435714 Human
see all -
別名
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
画像
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Western blot - Anti-PAK1 (phospho S204) antibody (ab79503)All lanes : Anti-PAK1 (phospho S204) antibody (ab79503) at 1/1000 dilution
Lane 1 : extracts from 3T3 cells (treated
with UV for 15 mins)
Lane 2 : extracts from 3T3 cells (treated
with UV for 15 mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 (phospho S204) antibody (ab79503)Immunohistochemistry analysis of paraffin-embedded human brain tissue, stained with ab79503, at 1/100 dilution, in the absence and presence of the immunising peptide
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab79503 は 1 報の論文で使用されています。
- Vaquié A et al. Injured Axons Instruct Schwann Cells to Build Constricting Actin Spheres to Accelerate Axonal Disintegration. Cell Rep 27:3152-3166.e7 (2019). PubMed: 31189102