Anti-PAK1 抗体 (ab183894)
Key features and details
- Rabbit polyclonal to PAK1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAK1 antibody
PAK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment within Human PAK1 aa 151-544. The exact sequence is proprietary.
Database link: Q13153 -
ポジティブ･コントロール
- Hepatoma tissue. A431 cells. HeLa, A431, BCL-1 and rat PC12 whole cell lysates/extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183894の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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IHC-P
1/100 - 1/1000. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated when activated by CDC42/p21 and RAC1. -
細胞内局在
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. - Information by UniProt
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参照データベース
- Entrez Gene: 533729 Cow
- Entrez Gene: 5058 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 29431 Rat
- Omim: 602590 Human
- SwissProt: Q08E52 Cow
- SwissProt: Q13153 Human
- SwissProt: O88643 Mouse
see all -
別名
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
画像
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Western blot - Anti-PAK1 antibody (ab183894)All lanes : Anti-PAK1 antibody (ab183894) at 1/5000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa -
Western blot - Anti-PAK1 antibody (ab183894)Anti-PAK1 antibody (ab183894) at 1/1000 dilution + BCL-1 whole cell lysate/extract at 30 µg
Predicted band size: 61 kDa -
Western blot - Anti-PAK1 antibody (ab183894)Anti-PAK1 antibody (ab183894) at 1/1000 dilution + rat PC12 whole cell lysate/extract at 30 µg
Predicted band size: 61 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody (ab183894)Immunohistochemical analysis of paraffin-embedded human hepatoma labeling PAK1 using ab183894 at a 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-PAK1 antibody (ab183894)Immunofluorescence analysis of A431 cells fixed in ice-cold methanol for 5 minutes, labeling PAK1 using ab183894 at a 1/500 dilution (green). Blue: Hoechst 33343 staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab183894 は論文での使用が確認できていません。