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リコンビナントRabMAb

Anti-PAIP1 抗体 [EPR13259] (ab181359)

Submit a review Submit a question References (2)
Western blot - Anti-PAIP1 antibody [EPR13259] (ab181359)
  • Immunoprecipitation - Anti-PAIP1 antibody [EPR13259] (ab181359)
  • Flow Cytometry (Intracellular) - Anti-PAIP1 antibody [EPR13259] (ab181359)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAIP1 antibody [EPR13259] (ab181359)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (ab181359)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (ab181359)
  • Anti-PAIP1 antibody [EPR13259] (ab181359)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13259] to PAIP1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PAIP1 antibody [EPR13259]
    PAIP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13259] to PAIP1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, HeLa, and 293T cell lysates, Human gastric carcinoma tissue, Human uterus tissue, HeLa cells, permeabilized 293 cells, immunoprecipitation pellet from HeLa whole cell lysate (ab150035)

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13259

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181359の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.

ターゲット情報

  • 機能

    Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.

  • 配列類似性

    Contains 1 MIF4G domain.

  • ドメイン

    Only the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • MGC12360 antibody
      • OTTHUMP00000221601 antibody
      • OTTMUSP00000026880 antibody
      • PABC1 interacting protein 1 antibody
      • PABP interacting protein 1 antibody
      • PABP-interacting protein 1 antibody
      • PAIP 1 antibody
      • PAIP-1 antibody
      • Paip1 antibody
      • PAIP1_HUMAN antibody
      • Poly(A) binding protein interacting protein 1 antibody
      • Poly(A)-binding protein-interacting protein 1 antibody
      • Polyadenylate binding protein interacting protein 1 antibody
      • Polyadenylate-binding protein-interacting protein 1 antibody
      see all

    画像

    • Western blot - Anti-PAIP1 antibody [EPR13259] (ab181359)
      Western blot - Anti-PAIP1 antibody [EPR13259] (ab181359)
      All lanes : Anti-PAIP1 antibody [EPR13259] (ab181359) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa

    • Immunoprecipitation - Anti-PAIP1 antibody [EPR13259] (ab181359)
      Immunoprecipitation - Anti-PAIP1 antibody [EPR13259] (ab181359)

      Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab181359 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    • Flow Cytometry (Intracellular) - Anti-PAIP1 antibody [EPR13259] (ab181359)
      Flow Cytometry (Intracellular) - Anti-PAIP1 antibody [EPR13259] (ab181359)

      Intracellular flow cytometric analysis of permeabilized 293 cells using ab181359 at 1/10 dilution (red) or a rabbit IgG (negative) (green). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-PAIP1 antibody [EPR13259] (ab181359)
      Immunocytochemistry/ Immunofluorescence - Anti-PAIP1 antibody [EPR13259] (ab181359)

      Immunofluorescence analysis of HeLa cells labeling PAIP1 with ab181359 at 1/50 dilution (green). DAPI nuclear staining (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (ab181359)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (ab181359)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling PAIP1 with ab181359 at 1/50 dilution.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (ab181359)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (ab181359)

      Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling PAIP1 with ab181359 at 1/50 dilution.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-PAIP1 antibody [EPR13259] (ab181359)
      Anti-PAIP1 antibody [EPR13259] (ab181359)

    参考文献 (2)

    ab181359 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181359 は 2 報の論文で使用されています。

    • Wang Y  et al. Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1. Reprod Sci 29:2647-2658 (2022). PubMed: 35378711
    • Xie H  et al. Effects of inducing apoptosis and inhibiting proliferation of siRNA on polyadenylate-binding protein-interacting protein 1 in tongue cell carcinoma. Head Neck 42:3623-3637 (2020). PubMed: 32827170

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