Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-PAI1 抗体 [EPR17796] (ab187263)

Submit a review Submit a question
Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263)
  • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
  • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263)
  • Immunoprecipitation - Anti-PAI1 antibody [EPR17796] (ab187263)
  • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
  • Anti-PAI1 antibody [EPR17796] (ab187263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17796] to PAI1
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PAI1 antibody [EPR17796]
    PAI1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17796] to PAI1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IP, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human PAI1 full length protein; A549, HUVEC, HepG2 whole cell lysate; Human fetal liver and fetal spleen lysates. ICC/IF: HepG2 and HT1080 cells. IP: HepG2 whole cell lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187263の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/500.
IP
1/40.
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
特記事項
ICC/IF
1/500.
IP
1/40.
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

  • 組織特異性

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

  • 関連疾患

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

  • 配列類似性

    Belongs to the serpin family.

  • 翻訳後修飾

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Clade E antibody
      • Endothelial plasminogen activator inhibitor antibody
      • Nexin antibody
      • Nexin plasminogen activator inhibitor type 1 antibody
      • PAI 1 antibody
      • PAI antibody
      • PAI-1 antibody
      • PAI1_HUMAN antibody
      • PLANH1 antibody
      • Plasminogen activator inhibitor 1 antibody
      • Plasminogen activator inhibitor type 1 antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serine proteinase inhibitor clade E member 1 antibody
      • serpin antibody
      • Serpin E1 antibody
      • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serpin peptidase inhibitor clade E antibody
      • Serpine 1 antibody
      • SERPINE1 antibody
      see all

    画像

    • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
      Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
      All lanes : Anti-PAI1 antibody [EPR17796] (ab187263) at 1/1000 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : SERPINE1 knockout A549 cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HEK-293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 45 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab187263 observed at 48 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

      ab187263 was shown to react with PAI1 in wild-type A549 cells in Western blot. The band observed in the edited lysate lane above 45 kDa is likely to represent SERPINE1 with an insertion. This has not been investigated further. Wild-type A549 and SERPINE1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab187263 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263)
      Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cells) cells labeling PAI1 with ab187263 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on HT1080 cells was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab187263 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
      Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
      All lanes : Anti-PAI1 antibody [EPR17796] (ab187263) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
      Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
      Anti-PAI1 antibody [EPR17796] (ab187263) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263)
      Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling PAI1 with ab187263 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on HepG2 cells was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab187263 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    • Immunoprecipitation - Anti-PAI1 antibody [EPR17796] (ab187263)
      Immunoprecipitation - Anti-PAI1 antibody [EPR17796] (ab187263)

      PAI1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab187263 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187263 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

      Lane 1: HepG2 whole cell lysate 10 µg (Input). Lane 2: ab187263 IP in HepG2 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187263 in HepG2 whole cell lysate.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 8 seconds.

    • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
      Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)
      Anti-PAI1 antibody [EPR17796] (ab187263) at 1/5000 dilution + Human PAI1 full length protein at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-PAI1 antibody [EPR17796] (ab187263)
      Anti-PAI1 antibody [EPR17796] (ab187263)

    参考文献 (0)

    ab187263 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187263 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab187263.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com