Anti-PAI1 抗体 (ab31280)
Key features and details
- Goat polyclonal to PAI1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAI1 antibody
PAI1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PAI1 -
由来種
Goat -
特異性
This antibody is expected to recognise both reported isoforms. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog, Chimpanzee
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免疫原
Synthetic peptide corresponding to Human PAI1 aa 50-150 (internal sequence).
Database link: NP_000593.1 -
ポジティブ･コントロール
- IHC-P: Human placenta tissue. WB: Human liver tissue, A549, HepG2, and HeLa cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31280の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
1 hour primary incubation is recommended for this product. |
| IHC-P | (1) |
Use a concentration of 4 - 6 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 4 - 6 µg/ml. |
ターゲット情報
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機能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
組織特異性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
関連疾患
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
配列類似性
Belongs to the serpin family. -
翻訳後修飾
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5054 Human
- Omim: 173360 Human
- SwissProt: Q7YSA1 Dog
- SwissProt: P05121 Human
- Unigene: 414795 Human
- Unigene: 713079 Human
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別名
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
画像
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Western blot - Anti-PAI1 antibody (ab31280)All lanes : Anti-PAI1 antibody (ab31280) at 0.1 µg/ml
Lane 1 : A549 cell lysate (35µg protein in RIPA buffer)
Lane 2 : HepG2 cell lysate (35µg protein in RIPA buffer)
Lane 3 : HeLa cell lysate (35µg protein in RIPA buffer)
Developed using the ECL technique.
Predicted band size: 45 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab31280)This image is courtesy of an anonymous Abreviewab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody.
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Western blot - Anti-PAI1 antibody (ab31280)Anti-PAI1 antibody (ab31280) at 0.3 µg/ml + Human liver lysate (35µg protein in RIPA buffer)
Developed using the ECL technique.
Predicted band size: 45 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab31280)ab31280 (4µg/ml) staining of paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab31280 は 8 報の論文で使用されています。
- Hogan TB et al. Caveolin-1 peptide regulates p53-microRNA-34a feedback in fibrotic lung fibroblasts. iScience 25:104022 (2022). PubMed: 35330685
- Chen Y et al. MiR145-5p inhibits proliferation of PMVECs via PAI-1 in experimental hepatopulmonary syndrome rat pulmonary microvascular hyperplasia. Biol Open 8:N/A (2019). PubMed: 31649116
- Luo M et al. Circulating miR-30c as a predictive biomarker of type 2 diabetes mellitus with coronary heart disease by regulating PAI-1/VN interactions. Life Sci 239:117092 (2019). PubMed: 31760103
- Tsuge M et al. SK-216, a Novel Inhibitor of Plasminogen Activator Inhibitor-1, Suppresses Lung Metastasis of Human Osteosarcoma. Int J Mol Sci 19:N/A (2018). PubMed: 29510576
- Hirahata M et al. PAI-1, a target gene of miR-143, regulates invasion and metastasis by upregulating MMP-13 expression of human osteosarcoma. Cancer Med 5:892-902 (2016). WB . PubMed: 26817521
- Falkenberg N et al. Secreted uPAR isoform 2 (uPAR7b) is a novel direct target of miR-221. Oncotarget 6:8103-14 (2015). WB . PubMed: 25797271
- Gillespie E et al. Plasminogen activator inhibitor-1 is increased in colonic epithelial cells from patients with colitis-associated cancer. J Crohns Colitis 7:403-11 (2013). PubMed: 22921465
- Keates AC et al. Helicobacter pylori infection stimulates plasminogen activator inhibitor 1 production by gastric epithelial cells. Infect Immun 76:3992-9 (2008). WB ; Human . PubMed: 18519558