Anti-PADI4 / PAD4 抗体 (ab50332)
Key features and details
- Rabbit polyclonal to PADI4 / PAD4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PADI4 / PAD4 antibody
PADI4 / PAD4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PADI4 / PAD4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to amino acids 1-15 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue
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ポジティブ・コントロール
- Extracts of DMSO-treated HL60 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50332の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 74 kDa.
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特記事項 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 74 kDa. |
ターゲット情報
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機能
Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. -
組織特異性
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. -
関連疾患
Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. -
配列類似性
Belongs to the protein arginine deiminase family. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils. - Information by UniProt
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参照データベース
- Entrez Gene: 23569 Human
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
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別名
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
画像
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All lanes : Anti-PADI4 / PAD4 antibody (ab50332) at 1/1000 dilution
Lane 1 : 293-T cells
Lane 2 : 293-T cells transfected with PADI4 cDNA
Lane 3 : HL60 cells
Lane 4 : HL60 cells induced with 1.25% DMSO for three days
Secondary
All lanes : Goat Anti-Rabbit IgG
Predicted band size: 74 kDa
Observed band size: ~62 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab50332 は 11 報の論文で使用されています。
- Mondal S et al. Site-specific incorporation of citrulline into proteins in mammalian cells. Nat Commun 12:45 (2021). PubMed: 33398026
- Uysal-Onganer P et al. Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures. Int J Mol Sci 22:N/A (2021). PubMed: 33573274
- D'Alessio S et al. Post-Translational Protein Deimination Signatures in Plasma and Plasma EVs of Reindeer (Rangifer tarandus). Biology (Basel) 10:N/A (2021). PubMed: 33805829
- Zheng Q et al. Synthesis of an Alkynyl Methylglyoxal Probe to Investigate Nonenzymatic Histone Glycation. J Org Chem 85:1691-1697 (2020). PubMed: 31875401
- Sancandi M et al. Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson's Disease. Int J Mol Sci 21:N/A (2020). PubMed: 32326590