AB181205

Anti-PACAP 抗体 [EPR11544]

Anti-PACAP antibody [EPR11544]

RabMAb
Recombinant
20ul selling size
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(12 Publications)

Rabbit Recombinant Monoclonal PACAP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 12 publications.

別名を表示する

MEDA7, PACAP, HSPC190, MZB1, Marginal zone B- and B1-cell-specific protein, Mesenteric estrogen-dependent adipose 7, Plasma cell-induced resident endoplasmic reticulum protein, Proapoptotic caspase adapter protein, MEDA-7, Plasma cell-induced resident ER protein, pERp1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (AB181205)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (AB181205)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PACAP with ab181205 at 1/250 dilution. The slide is counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (AB181205)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PACAP with ab181205 at 1/250 dilution. The slide is counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PACAP antibody [EPR11544] (AB181205)

All lanes:

Western blot - Anti-PACAP antibody [EPR11544] (ab181205) at 1/50000 dilution

Lane 1:

Raji cell lysate at 20 µg

Lane 2:

Ramos cell lysate at 20 µg

Lane 3:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR11544

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca(2+) stores, antibody secretion and integrin activation.. Acts as a hormone-regulated adipokine/pro-inflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.

See full target information MZB1

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:227 PubMed40001145

2025

Revolutionizing the treatment of intervertebral disc degeneration: an approach based on molecular typing.

Applications

Unspecified application

Species

Unspecified reactive species

Shaofeng Chen,Wei Zhang,Yifan Liu,Runzhi Huang,Xiaoyi Zhou,Xianzhao Wei

Frontiers in pharmacology 15:1442831 PubMed39206261

2024

Puerarin attenuates myocardial ischemic injury and endoplasmic reticulum stress by upregulating the Mzb1 signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaojiao Xue,Haolin Ren,Qi Zhang,Jing Gu,Qian Xu,Jiaxi Sun,Lu Zhang,Ming-Sheng Zhou

Journal of cellular and molecular medicine 27:3692-3705 PubMed37830980

2023

Dedifferentiated Schwann cells promote perineural invasion mediated by the PACAP paracrine signalling in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqiang Chen,Zhen Zheng,Hao Sun,Jiahao You,Jing Chu,Jinghai Gao,Lei Qiu,Xiaojun Liu

Nature communications 14:3958 PubMed37402769

2023

Whole genome sequencing identifies genetic variants associated with neurogenic inflammation in rosacea.

Applications

Unspecified application

Species

Unspecified reactive species

Zhili Deng,Mengting Chen,Zhixiang Zhao,Wenqin Xiao,Tangxiele Liu,Qinqin Peng,Zheng Wu,San Xu,Wei Shi,Dan Jian,Ben Wang,Fangfen Liu,Yan Tang,Yingxue Huang,Yiya Zhang,Qian Wang,Lunquan Sun,Hongfu Xie,Guohong Zhang,Ji Li

The Korean journal of pain 36:335-346 PubMed37394274

2023

Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/PKA/CREB signaling pathway in the trigeminal ganglion in rats.

Applications

Unspecified application

Species

Unspecified reactive species

QingLing Zhai,KaiXin Wang,Defu Zhang,Jinbo Chen,XiaoMeng Dong,Yonghui Pan

The Journal of general physiology 155: PubMed36538657

2022

PACAP and acetylcholine cause distinct Ca2+ signals and secretory responses in chromaffin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Morales,Ramkumar Mohan,Xiaohuan Chen,Breanna L Coffman,Mounir Bendahmane,Lester Watch,Joshua L West,Shreeya Bakshi,John R Traynor,David R Giovannucci,Paul J Kammermeier,Daniel Axelrod,Kevin P M Currie,Alan V Smrcka,Arun Anantharam

iScience 25:105366 PubMed36339258

2022

Single-cell RNA-seq transcriptomic landscape of human and mouse islets and pathological alterations of diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Chen,Junqing Zhang,Youyuan Huang,Xiaodong Tian,Yinmo Yang,Aimei Dong

Translational neuroscience 12:523-532 PubMed34963819

2021

Neuropeptide changes in an improved migraine model with repeat stimulations.

Applications

Unspecified application

Species

Unspecified reactive species

Yichen Guo,Yawen Cheng,Jiaqi An,Yi Qi,Guogang Luo

Genetics and molecular biology 44:e20200463 PubMed34807222

2021

Overexpression of RAPGEF3 enhances the therapeutic effect of dezocine in treatment of neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Liu,Li Song,Xiaojun Ma,Yong Liu,Hui Huang,Yongsheng Xu,Wei Yan

Free radical biology & medicine 174:236-248 PubMed34400297

2021

Pituitary adenylate cyclase-activating polypeptide attenuates mitochondria-mediated oxidative stress and neuronal apoptosis after subarachnoid hemorrhage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanjian Fang,Hui Shi,Lei Huang,Reng Ren,Cameron Lenahan,Jie Xiao,Yu Liu,Rui Liu,Rajvee Sanghavi,Chenguang Li,Sheng Chen,Jiping Tang,Jun Yu,John H Zhang,Jianmin Zhang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PACAP 抗体 [EPR11544]

Anti-PACAP antibody [EPR11544]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (AB181205)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (AB181205)

Western blot - Anti-PACAP antibody [EPR11544] (AB181205)

関連する標識済み抗体及び組成の異なる製品 (8)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (12)

Abcam product promise