AB216627

Anti-PACAP-38 抗体

Name: Anti-PACAP-38 抗体 (ab216627) | Abcam
Brand: Abcam Limited
SKU: AB216627
Price: 74000 JPY
Availability: InStock

Anti-PACAP-38 antibody

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(7 Publications)

Rabbit Polyclonal PACAP-38 antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ADCYAP1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

Pituitary adenylate cyclase-activating polypeptide, PACAP, ADCYAP1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (AB216627)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (AB216627)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue, labeling PACAP-38 using ab216627 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (AB216627)

Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse small intestine tissue, labeling PACAP-38 using ab216627 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (AB216627)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling PACAP-38 using ab216627 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human ADCYAP1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P18509

特異性

ab216627 is specific to the cleaved PACAP-38 chain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Pituitary adenylate cyclase-activating polypeptide-38 (PACAP-38) is a neuropeptide with key roles in the central and peripheral nervous systems. Another name is vasoactive intestinal peptide-related peptide. PACAP-38 is a 38-amino acid peptide and its molecular weight is approximately 4.5 kDa. It expresses widely notably in the hypothalamus pituitary gland and adrenal medulla. PACAP-38 interacts with specific receptors such as PAC1 and VPAC receptors to mediate its functions.

Biological function summary

PACAP-38 modulates various physiological processes through its signaling activities. It acts in neurotransmission neuroprotection and neurogenesis. In the immune system PACAP-38 contributes to anti-inflammatory responses by regulating cytokine production. It does not form part of a larger protein complex but exerts its effects independently via receptor binding serving important roles in maintaining homeostasis.

Pathways

PACAP-38 influences several signaling cascades. It is integral to the cAMP-PKA pathway which is important for regulating a variety of cellular processes including hormonal secretion and metabolic activity. Additionally PACAP-38 is involved in the MAPK/ERK pathway contributing to cell proliferation and survival. Related proteins such as the PAC1 receptor and adenylate cyclase play roles alongside PACAP-38 in these pathways.

PACAP-38 connects to migraine and schizophrenia. PACAP-38's role in dilation of cerebral arteries and its modulation of pain pathways link it to migraine where levels of this peptide can dictate disease manifestation. In schizophrenia altered PACAP-38 signaling affects neural circuits contributing to the disorder's symptoms. Through these disorders PACAP-38 interacts with proteins like calcitonin gene-related peptide (CGRP) in migraines and dopamine receptors in psychiatric conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

PACAP is a neuropeptide involved in diverse array of physiological processes through activating the PACAP subfamily of class B1 G protein-coupled receptors : VIP receptor 1 (VIPR1), VIP receptor 2 (VIPR2), and PACAP type I receptor (ADCYAP1R1) (PubMed : 11175907, PubMed : 23800469, PubMed : 32047270, PubMed : 36385145). Exerts neuroprotective and general cytoprotective effects due to anti-apoptotic, anti-inflammatory, and antioxidant actions (PubMed : 23800469). Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway (PubMed : 23800469). In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (By similarity). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity). PACAP exists in two bioactive forms from proteolysis of the same precursor protein, PACAP27 and PACAP38, which differ by eleven amino acid residues in the C-terminus (PubMed : 32047270).

See full target information ADCYAP1

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:35 PubMed39972389

2025

Spatial analysis of a complete DIPG-infiltrated brainstem reveals novel ligand-receptor mediators of tumour-to-TME crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Anja Kordowski,Onkar Mulay,Xiao Tan,Tuan Vo,Ulrich Baumgartner,Mellissa K Maybury,Timothy Hassall,Lachlan Harris,Quan Nguyen,Bryan W Day

CNS neuroscience & therapeutics 30:e14743 PubMed38780008

2024

Electroacupuncture regulates Rab5a-mediating NGF transduction to improve learning and memory ability in the early stage of AD mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhong Li,Minguang Yang,Yaling Dai,Xiaoqin Guo,Yanyi Ding,Xiaoling Li,Shenghang Zhang,Wenshan Xu,Lidian Chen,Jing Tao,Weilin Liu

International journal of molecular sciences 23: PubMed36555637

2022

Novel Small Molecule Positive Allosteric Modulator SPAM1 Triggers the Nuclear Translocation of PAC1-R to Exert Neuroprotective Effects through Neuron-Restrictive Silencer Factor.

Applications

Unspecified application

Species

Unspecified reactive species

Guangchun Fan,Shang Chen,Lili Liang,Huahua Zhang,Rongjie Yu

International journal of environmental research and public health 19: PubMed35627499

2022

Endocrine Disruptor Bisphenol a Affects the Neurochemical Profile of Nerve Fibers in the Aortic Arch Wall in the Domestic Pig.

Applications

Unspecified application

Species

Unspecified reactive species

Liliana Rytel,László Könyves,Slawomir Gonkowski

Translational psychiatry 10:396 PubMed33177511

2020

Early-life stress alters affective behaviors in adult mice through persistent activation of CRH-BDNF signaling in the oval bed nucleus of the stria terminalis.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Hu,Isabella Maita,Mimi L Phan,Edward Gu,Christopher Kwok,Andrew Dieterich,Mark M Gergues,Christine N Yohn,Yu Wang,Jiang-Ning Zhou,Xin-Rui Qi,Dick F Swaab,Zhiping P Pang,Paul J Lucassen,Troy A Roepke,Benjamin A Samuels

The Journal of neuroscience : the official journal 40:2519-2537 PubMed32054675

2020

Chronic Stress Induces Maladaptive Behaviors by Activating Corticotropin-Releasing Hormone Signaling in the Mouse Oval Bed Nucleus of the Stria Terminalis.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Hu,Ji Liu,Isabella Maita,Christopher Kwok,Edward Gu,Mark M Gergues,Frederric Kelada,Mimi Phan,Jiang-Ning Zhou,Dick F Swaab,Zhiping P Pang,Paul J Lucassen,Troy A Roepke,Benjamin A Samuels

Frontiers in neuroscience 13:1326 PubMed31920495

2019

Distinct VIP and PACAP Functions in the Distal Nerve Stump During Peripheral Nerve Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia K Woodley,Qing Min,Yankun Li,Nina F Mulvey,David B Parkinson,Xin-Peng Dun

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-PACAP-38 抗体

Anti-PACAP-38 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (AB216627)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (AB216627)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (AB216627)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

Abcam product promise