Anti-p95/NBS1 (phospho S343) 抗体 (ab47272)
Key features and details
- Rabbit polyclonal to p95/NBS1 (phospho S343)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-p95/NBS1 (phospho S343) antibody
p95/NBS1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p95/NBS1 (phospho S343) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human p95/NBS1 (phospho S343).
Database link: O60934 -
ポジティブ・コントロール
- Jurkat cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody against the non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47272の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 85 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 85 kDa). |
ターゲット情報
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機能
Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex. -
組織特異性
Ubiquitous. Expressed at high levels in testis. -
関連疾患
Nijmegen breakage syndrome
Breast cancer
Aplastic anemia
Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). -
配列類似性
Contains 1 BRCT domain.
Contains 1 FHA domain. -
ドメイン
The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response. -
翻訳後修飾
Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance. -
細胞内局在
Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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参照データベース
- Entrez Gene: 4683 Human
- Omim: 602667 Human
- SwissProt: O60934 Human
- Unigene: 492208 Human
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別名
- AT V1 antibody
- AT V2 antibody
- ATV antibody
see all
画像
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All lanes : Anti-p95/NBS1 (phospho S343) antibody (ab47272) at 1/500 dilution
Lane 1 : Unirradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate
Lane 2 : Irradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate
Lane 3 : Unirradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate
Lane 4 : Irradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat polyclonal to rabbit IgG at 1/3000 dilution
Predicted band size: 85 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab47272 は 11 報の論文で使用されています。
- Blanchard-Rohner G et al. Childhood-Onset Movement Disorders Can Mask a Primary Immunodeficiency: 6 Cases of Classical Ataxia-Telangiectasia and Variant Forms. Front Immunol 13:791522 (2022). PubMed: 35154108
- Herok M et al. Chemotherapy of HER2- and MDM2-Enriched Breast Cancer Subtypes Induces Homologous Recombination DNA Repair and Chemoresistance. Cancers (Basel) 13:N/A (2021). PubMed: 34572735
- Hollingworth R et al. Productive herpesvirus lytic replication in primary effusion lymphoma cells requires S-phase entry. J Gen Virol N/A:N/A (2020). PubMed: 32501196
- Weber AM et al. Phenotypic consequences of somatic mutations in the ataxia-telangiectasia mutated gene in non-small cell lung cancer. Oncotarget 7:60807-60822 (2016). WB . PubMed: 27602502
- Byrd PJ et al. A Hypomorphic PALB2 Allele Gives Rise to an Unusual Form of FA-N Associated with Lymphoid Tumour Development. PLoS Genet 12:e1005945 (2016). WB . PubMed: 26990772