Name: Anti-p73 antibody [EP436Y]
Brand: RabMAb
SKU: ab40658
Rating: 4 (1 reviews)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

Loading size & price…

Abpromise

保証された製品品質、優れたカスタマー・サポート。詳細はこちら。

Western blot - Anti-p73 antibody [EP436Y] (ab40658)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP436Y] to p73
Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
Reacts with: Mouse, Human

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

製品名

Anti-p73 antibody [EP436Y]
p73 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP436Y] to p73
由来種

Rabbit
アプリケーション

適用あり: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
適用なし: IP
種交差性

交差種: Mouse, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HeLa, Jurkat, HEK293 and NIH/3T3 cell lysates. IHC-P: Human urinary bladder carcinoma, human kidney, human liver carcinoma and mouse testis tissues. ICC/IF: HeLa cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP436Y
アイソタイプ

IgG
研究分野

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab40658の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/500 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa).
Flow Cyt (Intra)		Use at an assay dependent concentration.
ICC/IF		1/150 - 1/300.
IHC-P		1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

特記事項
WB 1/500 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa).
Flow Cyt (Intra) Use at an assay dependent concentration.
ICC/IF 1/150 - 1/300.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

追加情報

Is unsuitable for IP.

機能

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
組織特異性

Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
配列類似性

Belongs to the p53 family.
Contains 1 SAM (sterile alpha motif) domain.
ドメイン

Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
The WW-binding motif mediates interaction with WWOX.
翻訳後修飾

Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Ubiquitinated; leading to its degradation by the proteasome.
細胞内局在

Nucleus. Accumulates in the nucleus in response to DNA damage.
Target information above from: UniProt accession O15350 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 7161 Human
- Entrez Gene: 22062 Mouse
- Omim: 601990 Human
- SwissProt: O15350 Human
- SwissProt: Q9JJP2 Mouse
- Unigene: 697294 Human
- Unigene: 706990 Human
- Unigene: 78015 Mouse
別名
- p53 like transcription factor antibody
- p53 related protein antibody
- p53-like transcription factor antibody
- p53-related protein antibody
- p73 antibody
- P73_HUMAN antibody
- TP73 antibody
- Tumor protein p73 antibody
see all

Western blot - Anti-p73 antibody [EP436Y] (ab40658)

All lanes : Anti-p73 antibody [EP436Y] (ab40658) at 1/5000 dilution (purified)

Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 60,75 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-p73 antibody [EP436Y] (ab40658)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling p73 with purified ab40658 at a dilution of 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Flow Cytometry (Intracellular) - Anti-p73 antibody [EP436Y] (ab40658)

Intracellular Flow Cytometry analysis of 293 (human embryonic kidney) cells labeling p73 with purified ab40658 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Western blot - Anti-p73 antibody [EP436Y] (ab40658)

Anti-p73 antibody [EP436Y] (ab40658) at 1/1000 dilution (purified) + NIH/3T3 whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p73 antibody [EP436Y] (ab40658)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder carcinoma tissue labelling p73 with unpurified ab40658 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-p73 antibody [EP436Y] (ab40658)

All lanes : Anti-p73 antibody [EP436Y] (ab40658) at 1/2000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 70 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Anti-p73 antibody [EP436Y] (ab40658)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab40658 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab40658 は 63 報の論文で使用されています。

Riva M et al. Aberrant survival of hippocampal Cajal-Retzius cells leads to memory deficits, gamma rhythmopathies and susceptibility to seizures in adult mice. Nat Commun 14:1531 (2023). PubMed: 36934089
Zhu M et al. Effects of Follicular Fluid on Physiological Characteristics and Differentiation of Fallopian Tube Epithelial Cells Implicating for Ovarian Cancer Pathogenesis. Int J Mol Sci 24:N/A (2023). PubMed: 37373301
Strubel A et al. DARPins detect the formation of hetero-tetramers of p63 and p73 in epithelial tissues and in squamous cell carcinoma. Cell Death Dis 14:674 (2023). PubMed: 37828008
van Bruggen R et al. A Versatile Strategy for Genetic Manipulation of Cajal-Retzius Cells in the Adult Mouse Hippocampus. eNeuro 10:N/A (2023). PubMed: 37775311
Alhaddad L et al. Polyploid/Multinucleated Giant and Slow-Cycling Cancer Cell Enrichment in Response to X-ray Irradiation of Human Glioblastoma Multiforme Cells Differing in Radioresistance and TP53/PTEN Status. Int J Mol Sci 24:N/A (2023). PubMed: 36674747

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (hairy skin sections)

Specification

hairy skin sections

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.1% TRITON, 3'

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 07 2011

Question

I would very much like to use this antibody for IP for a ChIP experiment. Could you tell me why it is considered "unsuitable".

Abcam community

Verified customer

Asked on Aug 02 2013

Answer

ab40658 was tested in IP on 293T lysate, and detected a faint smear along with two non-specific bands. As a result, we didn't recommend it for use in IP.

Jeremy Kasanov

Abcam Scientific Support

Answered on Aug 02 2013

Question

One of our customers asks id there is cross reactivity with the delta-n isoform of the p73 protein

Abcam community

Verified customer

Asked on Nov 01 2011

Answer

The p73 antibody ab40658 should recognize all p73 isoforms, including the delta-n isoforms, except for isoform 10 according to the uniprot file below: http://www.uniprot.org/uniprot/O15350

Abcam Scientific Support

Answered on Nov 01 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (63)

レビューと Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-p73 antibody [EP436Y]