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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-P70 S6 Kinase beta/SRK 抗体 [EPR14956] - N-terminal (ab184551)

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Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
  • Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
  • Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
  • Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
  • Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
  • Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14956] to P70 S6 Kinase beta/SRK - N-terminal
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal
    P70 S6 Kinase beta/SRK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14956] to P70 S6 Kinase beta/SRK - N-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal liver lysate, Jurkat and K562 cell lysates, Human pancreas tissue. HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184551の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
特記事項
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.

ターゲット情報

  • 機能

    Phosphorylates specifically ribosomal protein S6.

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 70 kDa ribosomal protein S6 kinase 2 antibody
      • EC 2.7.11.1 antibody
      • KS6B2_HUMAN antibody
      • p70 beta antibody
      • p70 ribosomal S6 kinase beta antibody
      • p70 S6 kinase beta antibody
      • p70 S6K-beta antibody
      • p70 S6KB antibody
      • p70 S6Kbeta antibody
      • p70(S6K) beta antibody
      • p70-beta antibody
      • p70-S6K 2 antibody
      • P70S6K2 antibody
      • p70S6Kb antibody
      • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
      • Ribosomal protein S6 kinase B2 antibody
      • Ribosomal protein S6 kinase beta 2 antibody
      • Ribosomal protein S6 kinase beta-2 antibody
      • Rps6kb2 antibody
      • S6 kinase related kinase antibody
      • S6 kinase-related kinase antibody
      • S6K beta 2 antibody
      • S6K beta antibody
      • S6K-beta antibody
      • S6K-beta-2 antibody
      • S6K2 antibody
      • Serine/threonine protein kinase 14 beta antibody
      • Serine/threonine-protein kinase 14B antibody
      • SRK antibody
      • STK14B antibody
      see all

    画像

    • Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: RPS6KB2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Jurkat whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab184551 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab184551 was shown to specifically react with P70 S6 Kinase beta/SRK in wild-type HAP1 cells. No band was observed when RPS6KB2 knockout samples were used. Wild-type and RPS6KB2 knockout samples were subjected to SDS-PAGE.  Ab184551 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      Flow Cytometry (Intracellular) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)

      Intracellular Flow Cytometry analysis of2% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (green). Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      Immunocytochemistry/ Immunofluorescence - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)

      Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 488) secondary at a 1/200 dilution (green) costained with Dapi (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)

      Immunohistochemical analysis of paraffin-embedded Human pancreas labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/50 dilution, counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      All lanes : Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/50000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 53 kDa

    • Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/10000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 53 kDa

    • Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)
      Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551)

    参考文献 (13)

    ab184551 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184551 は 13 報の論文で使用されています。

    • Yang W  et al. CRNDE silencing promotes apoptosis and enhances cisplatin sensitivity of colorectal carcinoma cells by inhibiting the Akt/mTORC1-mediated Warburg effect. Oncol Lett 23:70 (2022). PubMed: 35069879
    • Zhang LJ  et al. miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1. Open Med (Wars) 16:1364-1371 (2021). PubMed: 34589612
    • Guo Y  et al. Spontaneous formation of neutrophil extracellular traps is associated with autophagy. Sci Rep 11:24005 (2021). PubMed: 34907287
    • Chen J  et al. Circ-GLI1 promotes metastasis in melanoma through interacting with p70S6K2 to activate Hedgehog/GLI1 and Wnt/ß-catenin pathways and upregulate Cyr61. Cell Death Dis 11:596 (2020). PubMed: 32732916
    • Zhang J & Fan J Prazosin inhibits the proliferation, migration and invasion, but promotes the apoptosis of U251 and U87 cells via the PI3K/AKT/mTOR signaling pathway. Exp Ther Med 20:1145-1152 (2020). PubMed: 32765662
    View all Publications for this product

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