Anti-p53R2 抗体 (ab8105)
Key features and details
- Rabbit polyclonal to p53R2
- Suitable for: WB, ICC, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-p53R2 antibody
p53R2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p53R2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide:
GDPERPEAAGLDQDER
, corresponding to amino acids 2-17 of Human p53R2. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8105の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
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| ICC |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 18997010
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| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). |
|
ICC
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 18997010 |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. -
組織特異性
Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. -
パスウェイ
Genetic information processing; DNA replication. -
関連疾患
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism. -
配列類似性
Belongs to the ribonucleoside diphosphate reductase small chain family. -
細胞内局在
Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 50484 Human
- Entrez Gene: 382985 Mouse
- Entrez Gene: 299976 Rat
- Omim: 604712 Human
- SwissProt: Q7LG56 Human
- SwissProt: Q6PEE3 Mouse
- Unigene: 512592 Human
- Unigene: 24738 Mouse
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別名
- DKFZp686M05248 antibody
- MGC102856 antibody
- MGC42116 antibody
see all
画像
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Western blot - Anti-p53R2 antibody (ab8105)All lanes : Anti-p53R2 antibody (ab8105) at 1 µg/ml
Lane 1 : 3T3 cell lysate with absence of blocking peptide
Lane 2 : 3T3 cell lysate with presence of blocking peptide
Predicted band size: 41 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab8105 は 27 報の論文で使用されています。
- Rudd SG et al. Ribonucleotide reductase inhibitors suppress SAMHD1 ara-CTPase activity enhancing cytarabine efficacy. EMBO Mol Med 12:e10419 (2020). PubMed: 31950591
- Zhang Q et al. miR-942 promotes proliferation and metastasis of hepatocellular carcinoma cells by inhibiting RRM2B. Onco Targets Ther 12:8367-8378 (2019). PubMed: 31632084
- Ding Y et al. Integrative Analysis Reveals Across-Cancer Expression Patterns and Clinical Relevance of Ribonucleotide Reductase in Human Cancers. Front Oncol 9:956 (2019). PubMed: 31637211
- Chen J et al. CD8+ tumor-infiltrating lymphocytes as a novel prognostic biomarker in lung sarcomatoid carcinoma, a rare subtype of lung cancer. Cancer Manag Res 10:3505-3511 (2018). PubMed: 30271199
- Fu Y et al. Nuclear RNR-a antagonizes cell proliferation by directly inhibiting ZRANB3. Nat Chem Biol 14:943-954 (2018). PubMed: 30150681