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AB17990

Anti-p53 抗体

Anti-p53 antibody

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(12 Publications)

Rabbit Polyclonal P53 antibody. Suitable for IHC-P, IP, Flow Cyt, Flow Cyt (Intra), WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human TP53 aa 50-100.

別名を表示する

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody (AB17990)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody (AB17990)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling p53 with ab17990 at 1/5000 dilution. Detection : DAB.

Flow Cytometry (Intracellular) - Anti-p53 antibody (AB17990)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-p53 antibody (AB17990)

ab17990 at 1.5ug/ml staining p53 in human Jurkat cell line by Flow Cytometery. Cells were fixed in 1.5% paraformaldehyde, and permeabilized in 90% methanol. A FITC-conjugated goat anti rabbit was used as secondary. The black line indicates control anti KLH IgG and the red line repersents ab17990 staining.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, IP, Flow Cyt (Intra), WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human TP53 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P04637

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代替製品

Primary Antibodies

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組合せ製品

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1.5 µg for 10^5 Cells", "FlowCyt-species-notes": "<p>1.5 μg per 1 million cells in a 150 μl reaction.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1.5 µg for 10^5 Cells", "FlowCytIntra-species-notes": "<p>1.5 μg per 1 million cells in a 150 μl reaction.</p><p><a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 35618207, PubMed : 36634798, PubMed : 38653238, PubMed : 9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17189187, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 38653238, PubMed : 9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed : 12524540, PubMed : 17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed : 12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed : 12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).
See full target information TP53
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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40141421

2025

PAR Serves an Indispensable Role in Controlling PAR Oncogenicity: The β-Catenin-p53 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanga Appasamy,Jeetendra Kumar Nag,Hodaya Malka,Rachel Bar-Shavit

Journal of nanobiotechnology 21:117 PubMed37005668

2023

Precise pancreatic cancer therapy through targeted degradation of mutant p53 protein by cerium oxide nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Wang Zhang,Bochuan Hu,Xiaohua Qin,Tianxiang Yi,Yayi Ye,Xiaowan Huang,Yang Song,Zhenyu Yang,Jieying Qian,Yunjiao Zhang

EMBO molecular medicine 12:e11466 PubMed32150356

2020

A paracrine activin A-mDia2 axis promotes squamous carcinogenesis via fibroblast reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Cangkrama,Mateusz Wietecha,Nicolas Mathis,Rin Okumura,Luca Ferrarese,Dunja Al-Nuaimi,Maria Antsiferova,Reinhard Dummer,Metello Innocenti,Sabine Werner

Investigational new drugs 38:634-649 PubMed31240514

2019

Tetra-substituted pyrrole derivatives act as potent activators of p53 in melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Chiara Proto,Donatella Fiore,Giovanni Forte,Paola Cuozzo,Anna Ramunno,Caterina Fattorusso,Patrizia Gazzerro,Maria Pascale,Silvia Franceschelli

Gene 712:143944 PubMed31233763

2019

LncRNA PLAC2 upregulates p53 to induce hepatocellular carcinoma cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujian Zheng,Pengxiang Lv,Shaoping Wang,Qing Cai,Bao Zhang,Feng Huo

Gene 711:143939 PubMed31220581

2019

Inhibition of sirtuin 1 deacetylase by miR-211-5p provides a mechanism for the induction of cell death in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sahar Yarahmadi,Zohreh Abdolvahabi,Zahra Hesari,Masoumeh Tavakoli-Yaraki,Zeynab Yousefi,Parvaneh Seiri,Saman Hosseinkhani,Mitra Nourbakhsh

Experimental and therapeutic medicine 16:4193-4200 PubMed30344694

2018

Sonic hedgehog signaling regulates hypoxia/reoxygenation-induced H9C2 myocardial cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Ying Zhang,Zeng-Yong Qiao,Hua-Jin Liu,Jiang-Wei Ma

Cell chemical biology 25:761-774.e5 PubMed29681526

2018

A Designed Peptide Targets Two Types of Modifications of p53 with Anti-cancer Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Lunxi Liang,Huanbin Wang,Hubing Shi,Zhaoli Li,Han Yao,Zhigao Bu,Ningning Song,Chushu Li,Dabin Xiang,Yao Zhang,Jilin Wang,Ye Hu,Qi Xu,Yanlei Ma,Zhongyi Cheng,Yingchao Wang,Shuliang Zhao,Jin Qian,Yingxuan Chen,Jing-Yuan Fang,Jie Xu

Scientific reports 7:40960 PubMed28102346

2017

Human p53 interacts with the elongating RNAPII complex and is required for the release of actinomycin D induced transcription blockage.

Applications

ChIP, IP

Species

Human, Human

Barbara N Borsos,Ildikó Huliák,Hajnalka Majoros,Zsuzsanna Ujfaludi,Ákos Gyenis,Peter Pukler,Imre M Boros,Tibor Pankotai

BMC cancer 15:644 PubMed26400193

2015

Expression profiling of O(6) methylguanine-DNA-methyl transferase in prolactinomas: a correlative study of promoter methylation and pathological features in 136 cases.

Applications

IHC

Species

Unspecified reactive species

Xiao-Bing Jiang,Bin Hu,Dong-Sheng He,Zhi-Gang Mao,Xin Wang,Bing-Bing Song,Yong-Hong Zhu,Hai-Jun Wang
View all publications
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