Anti-p38 (phospho Y182) 抗体 (ab47363)
Key features and details
- Rabbit polyclonal to p38 (phospho Y182)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-p38 (phospho Y182) antibody
p38 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p38 (phospho Y182) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human p38 (phospho Y182).
Database link: Q16539 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47363の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. -
組織特異性
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1432 Human
- Omim: 600289 Human
- SwissProt: Q16539 Human
- Unigene: 485233 Human
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別名
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
see all
画像
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Western blot - Anti-p38 (phospho Y182) antibody (ab47363)All lanes : Anti-p38 (phospho Y182) antibody (ab47363)
Lane 1 : K562 cells, treated with UV
Lane 2 : K562 cells, untreated
Predicted band size: 41 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho Y182) antibody (ab47363)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using ab47363. Left hand image without immunising peptide; right hand image with immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (126)
ab47363 は 126 報の論文で使用されています。
- Han J et al. Sesquiterpene lactones-enriched fractions from Xanthium mongolicum Kitag alleviate RA by regulating M1 macrophage polarization via NF-κB and MAPK signaling pathway. Front Pharmacol 14:1104153 (2023). PubMed: 36778009
- Jin R et al. Porphyromonas gingivalis-induced periodontitis could contribute to cognitive impairment in Sprague-Dawley rats via the P38 MAPK signaling pathway. Front Cell Neurosci 17:1141339 (2023). PubMed: 37056710
- Qi Z et al. Protection of toll-like receptor 9 against lipopolysaccharide-induced inflammation and oxidative stress of pulmonary epithelial cells via MyD88-mediated pathways. Physiol Res 71:259-273 (2022). PubMed: 35275698
- Yang Z et al. Metal Ion Periplasmic-Binding Protein YfeA of Glaesserella parasuis Induces the Secretion of Pro-Inflammatory Cytokines of Macrophages via MAPK and NF-κB Signaling through TLR2 and TLR4. Int J Mol Sci 23:N/A (2022). PubMed: 36077023
- Yang D et al. Sinensetin attenuates oxygen-glucose deprivation/reperfusion-induced neurotoxicity by MAPK pathway in human cerebral microvascular endothelial cells. J Appl Toxicol 42:683-693 (2022). PubMed: 34664717