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リコンビナントRabMAb

Anti-p38 (phospho T180) 抗体 [EPR16587] (ab178867)

Reviews (2) Submit a question References (68)
Western blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
  • Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
  • Flow Cytometry (Intracellular) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
  • Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
  • Indirect ELISA - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
  • Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16587] to p38 (phospho T180)
  • Suitable for: Flow Cyt (Intra), Dot blot, WB, IHC-P, Indirect ELISA
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-p38 (phospho T180) antibody [EPR16587]
    p38 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR16587] to p38 (phospho T180)

  • 由来種

    Rabbit

  • 特異性

    ab178867 recognizes alpha, beta, gamma and delta isoforms of (T180) phospho p38. ab178867 weakly cross-reacts with phosphorylated Y182.

  • アプリケーション

    適用あり: Flow Cyt (Intra), Dot blot, WB, IHC-P, Indirect ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa treated with anisomycin whole cell lysates. IHC-P: Human squamous cell cervical carcinoma and transitional cell carcinoma of bladder tissues. Flow Cyt (intra): Jurkat cells treated with anisomycin.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab178867の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/340.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Dot blot
1/1000.
WB (2)
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Indirect ELISA
Use at an assay dependent concentration.
特記事項
Flow Cyt (Intra)
1/340.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Dot blot
1/1000.
WB
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Indirect ELISA
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

  • 組織特異性

    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

  • 配列類似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.

  • ドメイン

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

  • 翻訳後修飾

    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CSAID Binding Protein 1 antibody
      • CSAID binding protein antibody
      • CSAID-binding protein antibody
      • Csaids binding protein antibody
      • CSBP 1 antibody
      • CSBP 2 antibody
      • CSBP antibody
      • CSBP1 antibody
      • CSBP2 antibody
      • CSPB1 antibody
      • Cytokine suppressive anti-inflammatory drug-binding protein antibody
      • EXIP antibody
      • MAP kinase 14 antibody
      • MAP kinase MXI2 antibody
      • MAP kinase p38 alpha antibody
      • MAPK 14 antibody
      • MAPK14 antibody
      • MAX interacting protein 2 antibody
      • MAX-interacting protein 2 antibody
      • Mitogen Activated Protein Kinase 14 antibody
      • Mitogen activated protein kinase p38 alpha antibody
      • Mitogen-activated protein kinase 14 antibody
      • Mitogen-activated protein kinase p38 alpha antibody
      • MK14_HUMAN antibody
      • Mxi 2 antibody
      • MXI2 antibody
      • p38 ALPHA antibody
      • p38 antibody
      • p38 MAP kinase antibody
      • p38 MAPK antibody
      • p38 mitogen activated protein kinase antibody
      • p38ALPHA antibody
      • p38alpha Exip antibody
      • PRKM14 antibody
      • PRKM15 antibody
      • RK antibody
      • SAPK2A antibody
      • Stress-activated protein kinase 2a antibody
      see all

    画像

    • Western blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Western blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Lane 1 : Anti-p38 (phospho T180) antibody [EPR16587] (ab178867) at 1/1000 dilution
      Lane 2 : Anti-p38 (phospho T180) antibody [EPR16587] (ab178867) at 1/10000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates treated with 250ng/ml anisomycin for 30 minutes
      Lane 2 : Untreated Hela whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

      Dot blot analysis of p38 peptides using ab178867 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST.

      Lane 1: Single phospho peptide pT180
      Lane 2: Single phospho peptide pY182
      Lane 3: Double phospho peptide pT180/pY182
      Lane 4: Non-phospho peptide

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

      Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma tissue labeling p38 (phospho T180) with ab178867 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of cervix is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

      Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling p38 (phospho T180) with ab178867 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear with additional cytoplasmic staining on cancer cells of bladder is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Flow Cytometry (Intracellular) - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with Anisomycin (250ng/ml for 30min)labeling p38 (phospho T180) with ab178867 at 1/340 dilution followed byGoat anti rabbit IgG (FITC) at 1/150 (red). 

      Controls:- 

      Blue - Unlabelled (Cells without incubation with primary antibody and secondary antibody) 

      Black - Rabbit monoclonal IgG Isotype control 

      Orange - Jurkat cells treated with Anisomycin (250ng/ml for 30min) labeling p38 (phospho T180) with ab178867 at 1/340preincubated with 1mg/ml p38 (phospho T180) peptide 

       

       

    • Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Dot Blot - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

      Dot blot analysis of p38 (phospho T180) using ab178867 at 1:2000 (1 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution. Exposure time: 3 minutes. Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Lane 1: MPK11/beta (T180) phospho peptide
      Lane 2: MPK11/beta non-phospho peptide
      Lane 3: MPK12/gamma (T183) phospho peptide
      Lane 4: MPK12/gamma non-phospho peptide
      Lane 5: MPK13/delta (T180) phospho peptide
      Lane 6: MPK13/delta non-phospho peptide
      Lane 7: MPK14/alpha (T180) phospho peptide
      Lane 8: MPK14/alpha non-phospho peptide

    • Indirect ELISA - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Indirect ELISA - Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

      Indirect ELISA showing primary antibody ab178867 binding to Human MPK11, MPK12, MPK13, and MPK14 phosphorylated and non-phosphorylated peptides. Antigen concentration is 100 ng/ml.

      Binding of ab178867 was assessed in a serial dilution range 1000-0 ng/ml.

      Binding was detected using the secondary antibody, Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    • Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)
      Anti-p38 (phospho T180) antibody [EPR16587] (ab178867)

    参考文献 (68)

    ab178867 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab178867 は 68 報の論文で使用されています。

    • Zha Y  et al. ADAMTS8 Promotes Cardiac Fibrosis Partly Through Activating EGFR Dependent Pathway. Front Cardiovasc Med 9:797137 (2022). PubMed: 35224040
    • Chang ZY  et al. Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35269806
    • Guo G & Dong J Diosmetin attenuates oxidative stress-induced damage to lens epithelial cells via the mitogen-activated protein kinase (MAPK) pathway. Bioengineered 13:11072-11081 (2022). PubMed: 35481411
    • Yu D  et al. Quercitrin protects human bronchial epithelial cells from oxidative damage. Open Med (Wars) 17:375-383 (2022). PubMed: 35799602
    • Liang ZH  et al. Exosomes from mmu_circ_0001052-modified adipose-derived stem cells promote angiogenesis of DFU via miR-106a-5p and FGF4/p38MAPK pathway. Stem Cell Res Ther 13:336 (2022). PubMed: 35870977
    View all Publications for this product

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-p38 (phospho T180) antibody [EPR16587]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    UV (100 J/m2)
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 12 2018

    Western blot abreview for Anti-p38 (phospho T180) antibody [EPR16587]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Loading amount
    1e+006 cells
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 23 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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