Anti-p38 (phospho T180 + Y182) 抗体 (ab4822)
Key features and details
- Rabbit polyclonal to p38 (phospho T180 + Y182)
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-p38 (phospho T180 + Y182) antibody
p38 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p38 (phospho T180 + Y182) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Dog, Carp, Monkey -
免疫原
-
ポジティブ・コントロール
- WB: HeLa, A431, COLO 205, A549 and A549 cell lysate; HEK-293 (human epithelial cell line from embryonic kidney) cells. IHC-P: Human brain tissue, human heart tissue, rat heart tissue. ICC: SH-SY5Y (human neuroblastoma cell line from bone marrow) cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free. PBS without Mg2+ and Ca2+. -
Concentration information loading...
-
精製度
Protein A purified -
特記事項(精製)
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38, and ii) a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4822の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/10 - 1/100.
|
|
ICC/IF |
1/250.
|
|
WB | (3) |
1/1000. Predicted molecular weight: 38 kDa.
|
特記事項 |
---|
IHC-P
1/10 - 1/100. |
ICC/IF
1/250. |
WB
1/1000. Predicted molecular weight: 38 kDa. |
ターゲット情報
-
機能
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. -
組織特異性
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 403856 Dog
- Entrez Gene: 1432 Human
- Entrez Gene: 26416 Mouse
- Entrez Gene: 81649 Rat
- Omim: 600289 Human
- SwissProt: O02812 Dog
- SwissProt: Q16539 Human
- SwissProt: P47811 Mouse
see all -
別名
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
see all
画像
-
All lanes : Anti-p38 (phospho T180 + Y182) antibody (ab4822) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) exposed for 40 min with UV, cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) exposed for 40 min with UV, cell lysate
Lane 5 : COLO 205 (human colon adenocarcinoma cell line) cell lysate
Lane 6 : COLO 205 (human colon adenocarcinoma cell line) exposed for 40 min with UV, cell lysate
Lane 7 : A549 (human lung carcinoma cell line) cell lysate
Lane 8 : A549 (human lung carcinoma cell line) exposed for 40 min with UV, cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
Paraffin-embedded human brain tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/100 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
Paraffin-embedded human heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
Paraffin-embedded rat heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
-
4% PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling p38 (phospho T180 + Y182) (Panel A: green) using ab4822 at 1 µg/mL in ICC/IF. Secondary antibody: Alexa Flour® 488 Goat Anti-Rabbit IgG at 1/400 dilution. Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control.
-
Peptide Competition: Extracts prepared from HEK-293 (human epithelial cell line from embryonic kidney) cells treated with UV irradiation were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4822 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphorylated peptide derived from the corresponding region of JNK 1 & 2 (6), and, the phosphorylated peptide derived from the corresponding region of ERK 1 & 2 (7). After washing, membranes were incubated with goat F(ab')2 antirabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method. The data show that only the phosphopeptide
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (192)
ab4822 は 192 報の論文で使用されています。
- Nighot M et al. Long-Term Use of Proton Pump Inhibitors Disrupts Intestinal Tight Junction Barrier and Exaggerates Experimental Colitis. J Crohns Colitis 17:565-579 (2023). PubMed: 36322638
- da C Fernandes CJ et al. Epigenetic Differences Arise in Endothelial Cells Responding to Cobalt-Chromium. J Funct Biomater 14:N/A (2023). PubMed: 36976051
- Zhang YY et al. Levetiracetam attenuates diabetes-associated cognitive impairment and microglia polarization by suppressing neuroinflammation. Front Pharmacol 14:1145819 (2023). PubMed: 37214458
- Ding J et al. The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction. PLoS One 18:e0286903 (2023). PubMed: 37310976
- Zhang H et al. Immunomodulatory activity of semen Ziziphi Spinosae protein: a potential plant protein functional food raw material. NPJ Sci Food 7:32 (2023). PubMed: 37336871