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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 抗体 [Y122] (ab32142)

Submit a review Q&A (1)References (43)
Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
  • Immunocytochemistry/ Immunofluorescence - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
  • Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y122] to p38 beta/MAPK11 + p38 alpha/MAPK14
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122]

  • 製品の詳細

    Rabbit monoclonal [Y122] to p38 beta/MAPK11 + p38 alpha/MAPK14

  • 由来種

    Rabbit

  • 特異性

    This antibody recognises p38. It is predicted to react with splice isoform CSBP1 according to sequence homology. Mouse cross reactivity has been tested by WB and IHC, Rat cross reactivity by WB only.

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Hela, Jurkat, HEK-293T, K562 and MCF7 cell lyastes. IHC-P: Human skin carcinoma. ICC/IF: MCF7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32142の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

画像

  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution

    Lane 1 : MAPK11 recombinant (ab117219)
    Lane 2 : MAPK12 recombinant (ab117221)
    Lane 3 : MAPK13 recombinant (ab113869)
    Lane 4 : MAPK14 (p38) recombinant (ab82188)

    Lysates/proteins at 0.5 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Green - ab32142 observed at 43 kDa.

    ab32142 was shown to react with Anti-p38 antibody [Y122] in Western blot. Membranes were blocked in 100% Licor before incubation with ab32142 and overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution

    Lane 1 : Recombinant Human p38 beta/MAPK11 protein (ab117219)
    Lane 2 : Recombinant Human p38 gamma/MAPK12 protein (ab117221)
    Lane 3 : Recombinant Human p38 delta/MAPK13 protein (ab113869)
    Lane 4 : Recombinant Human p38 alpha/MAPK14 protein (ab82188)

    Predicted band size: 41 kDa

  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : MAPK14 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 41 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab130007 observed at 125 kDa.  

     ab32142 was shown to react with p38 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255406 (knockout cell lysate ab263787) was used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : p38 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 41 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32142 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)  secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Immunocytochemistry/ Immunofluorescence - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling p38 (green) with purified ab32142 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)

    Ab32142, at a 1/100 dilution, staining p38 in paraffin embedded human skin carcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : p38 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 41 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab32142 and a competitor's top cited rabbit polyclonal antibody.

  • Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Western blot - Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    All lanes : Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142) at 1/50000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : MCF7 cell lysate

    Predicted band size: 41 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    10 ug protein per lane
  • Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)
    Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] (ab32142)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (43)

ab32142 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32142 は 43 報の論文で使用されています。

  • Le X  et al. Efficacy of Baishao Luoshi decoction on synaptic plasticity in rats with post stroke spasticity. J Tradit Chin Med 43:295-302 (2023). PubMed: 36994517
  • Chen Y  et al. PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. FEBS J 289:3839-3853 (2022). PubMed: 35080339
  • Zhuang Q  et al. Knockdown of circ-RAD23B inhibits non-small cell lung cancer progression via the miR-142-3p/MAP4K3 axis. Thorac Cancer 13:750-760 (2022). PubMed: 35106926
  • Song XY  et al. Cangxitongbi capsules protect the articular cartilage in the rat knee through the long non-coding RNA HOTAIR/p38MAPK pathway. Ann Transl Med 10:23 (2022). PubMed: 35242868
  • Zhang Y  et al. FimH confers mannose-targeting ability to Bacillus Calmette-Guerin for improved immunotherapy in bladder cancer. J Immunother Cancer 10:N/A (2022). PubMed: 35361729
View all Publications for this product

レビューと Q&A

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Question

 I would like to test ab32142 in canine FFPE tissue IHC  

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Answer

DISCOUNT CODE: *** Expiration date: April 20th, 2012 I am very pleased to hear you would like to accept our offer and test ab32142 in canine. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for canine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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