Anti-P2Y12 抗体 [EPR26298-93] - BSA and Azide free (ab300141)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue.
Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse spinal cord.
Panel B: anti-GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C: anti-P2RY12 staining microglia in mouse spinal cord.
Panel D: anti-FMR1 staining neurons in mouse spinal cord.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on rat spinal cord.
Panel B: anti-GPR17 staining oligodendrocytes in rat spinal cord.
Panel C: anti-P2RY12 staining microglia in rat spinal cord.
Panel D: anti-GFAP staining astrocytes in rat spinal cord.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse spinal cord.
Panel B: anti-GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C: anti-P2RY12 staining microglia in mouse spinal cord.
Panel D: anti-GFAP staining astrocytes in mouse spinal cord.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse hippocampus.
Panel B: anti-GPR17 staining oligodendrocytes in mouse hippocampus.
Panel C: anti-P2RY12 staining microglia in mouse hippocampus.
Panel D: anti-GFAP staining astrocytes in mouse hippocampus.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse cerebellum.
Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebellum.
Panel C: anti-P2RY12 staining microglia in mouse cerebellum.
Panel D: anti-GFAP staining astrocytes in mouse cerebellum.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300141, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat hippocampus tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on rat hippocampus.
Panel B: anti-GPR17 staining oligodendrocytes in rat hippocampus.
Panel C: anti-P2RY12 staining microglia in rat hippocampus.
Panel D: anti-FMR1 staining neurons in rat hippocampus.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on rat cerebrum.
Panel B: anti-GPR17 staining oligodendrocytes in rat cerebrum.
Panel C: anti-P2RY12 staining microglia in rat cerebrum.
Panel D: anti-FMR1 staining neurons in rat cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining: in the order of ab316105 at 1/500 dilution, ab300140 at 1/40000 dilution , and ab259335 at 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstaining with DAPI.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse hippocampus.
Panel B: anti-GPR17 staining oligodendrocytes in mouse hippocampus.
Panel C: anti-P2RY12 staining microglia in mouse hippocampus.
Panel D: anti-FMR1 staining neurons in mouse hippocampus.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining: in the order of ab316105 at 1/500 dilution, ab300140 at 1/40000 dilution, and ab259335 at 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on rat cerebrum.
Panel B: anti-GPR17 staining oligodendrocytes in rat cerebrum.
Panel C: anti-P2RY12 staining microglia in rat cerebrum.
Panel D: anti-GFAP staining astrocytes in rat cerebrum.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/140000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse cerebrum.
Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebrum.
Panel C: anti-P2RY12 staining microglia in mouse cerebrum.
Panel D: anti-FMR1 staining neurons in mouse cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse cerebrum.
Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebrum.
Panel C: anti-P2RY12 staining microglia in mouse cerebrum.
Panel D: anti-GFAP staining astrocytes in mouse cerebrum.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/140000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-FMR1 (gray; Opal™570) on mouse cerebellum.
Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebellum.
Panel C: anti-P2RY12 staining microglia in mouse cerebellum.
Panel D: anti-FMR1 staining neurons and Purkinje cells in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution and ab259335 at a 1/10000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab300140, the same antibody clone in a different buffer formulation.

This data was developed using ab300140, the same antibody clone in a different buffer formulation. 

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin. 

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Positive staining on microglial cells in human cerebrum (PMID: 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. 

This data was developed using ab300140, the same antibody clone in a different buffer formulation. 

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin. 

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

Positive staining on microglial cells in mouse cerebrum (PMID: 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. 

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

This data was developed using ab300140, the same antibody clone in a different buffer formulation. 

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin. 

Heat mediated antigen retrieval was perormed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

Positive staining on microglial cells in rat cerebrum (PMID: 30196821). The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. 

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

This data was developed using ab300140, the same antibody clone in a different buffer formulation. 

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling P2Y12 with ab300140 at 1/40000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin. 

Heat mediated antigen retrieval was performedwith Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

Negative control: no staining on human liver. The section was incubated with ab300140 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

This data was developed using ab300140, the same antibody clone in a different buffer formulation. 

Immunohistological analysis of 4% PFA fixed and 0.2% Triton X-100 parmeabilized frozen rat cerebrum (fresh) tissue labeling P2Y12 with ab300140 at 1/500 dilution, followed by (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. DAPI was used as nuclear counterstain. 

Positive staining on rat cerebrum. 

Secondary antibody only control used PBS instead of primary antibody, followed by secondary (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. 

This data was developed using ab300140, the same antibody clone in a different buffer formulation. 

Immunohistological analysis of 4% PFA fixed and 0.2% Triton X-100 parmeabilized frozen mouse cerebrum (fresh) tissue labeling P2Y12 with ab300140 at 1/500 dilution, followed by (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. DAPI was used as nuclear counterstain. 

Positive staining on mouse cerebrum. 

Secondary antibody only control used PBS instead of primary antibody, followed by secondary (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.