Anti-P2Y12 抗体 [EPR18611] (ab184411)
![Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (ab184411)](https://www.abcam.co.jp/ps/products/184/ab184411/Images/ab184411-252642-anti-p2y12-antibody-epr18611-immunoprecipitation.jpg "Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (ab184411)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18611] to P2Y12
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-P2Y12 antibody [EPR18611]
P2Y12 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18611] to P2Y12 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; mouse and rat brain tissue lysates. IP: Rat brain whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18611 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184411の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
1/80.
|
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| WB |
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
|
| 特記事項 |
|---|
|
IP
1/80. |
|
WB
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation. -
組織特異性
Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain. -
関連疾患
Defects in P2RY12 are the cause of P2RY12 deficiency (P2RY12D) [MIM:609821]. It is a condition characterized by severe impairment of platelet response to ADP and abnormal bleeding marked by excessive posttraumatic and postsurgical blood loss. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 70839 Mouse
- Entrez Gene: 64803 Rat
- SwissProt: Q9CPV9 Mouse
- SwissProt: Q9EPX4 Rat
- Unigene: 422675 Mouse
- Unigene: 64592 Rat
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別名
- ADP glucose receptor antibody
- ADP-glucose receptor antibody
- ADPG R antibody
see all
画像
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Immunoprecipitation - Anti-P2Y12 antibody [EPR18611] (ab184411)
P2Y12 was immunoprecipitated from 1mg of Rat brain whole cell lysate with ab184411 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab184411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Rat brain whole cell lysate 10µg (Input).
Lane 2: ab184411 IP in Rat brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184411 in Rat brain whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411)](https://www.abcam.co.jp/ps/products/184/ab184411/Images/ab184411-252644-anti-p2y12-antibody-epr18611-western-blot.jpg)
Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411)All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411)](https://www.abcam.co.jp/ps/products/184/ab184411/Images/ab184411-252643-anti-p2y12-antibody-epr18611-western-blot.jpg)
Western blot - Anti-P2Y12 antibody [EPR18611] (ab184411)All lanes : Anti-P2Y12 antibody [EPR18611] (ab184411) at 1/2000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-P2Y12 antibody [EPR18611] (ab184411)](https://www.abcam.co.jp/ps/products/184/ab184411/Images/ab184411-6-benefits-of-recombinant-antibodies.png)
Anti-P2Y12 antibody [EPR18611] (ab184411)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (13)
ab184411 は 13 報の論文で使用されています。
- Yang X et al. GSDMD knockdown attenuates phagocytic activity of microglia and exacerbates seizure susceptibility in TLE mice. J Neuroinflammation 20:193 (2023). PubMed: 37612735
- Liu S et al. Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system. Commun Biol 6:1239 (2023). PubMed: 38066234
- Sun ZQ et al. Lycium barbarum extract promotes M2 polarization and reduces oligomeric amyloid-β-induced inflammatory reactions in microglial cells. Neural Regen Res 17:203-209 (2022). PubMed: 34100457
- Jiang LP et al. Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice. Br J Pharmacol 179:46-64 (2022). PubMed: 34415054
- Tang G et al. Naringin Relieves Diabetic Cardiac Autonomic Neuropathy Mediated by P2Y14 Receptor in Superior Cervical Ganglion. Front Pharmacol 13:873090 (2022). PubMed: 35529431