Anti-P2X7 抗体 (ab109054)
Key features and details
- Rabbit polyclonal to P2X7
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-P2X7 antibody
P2X7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to P2X7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in Rat Hippocampus tissue lysate in western blot, and FFPE rat brain tissue sections in IHCP.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109054の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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| IHC-P |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
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IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. -
組織特異性
Widely expressed with highest levels in brain and immune tissues. -
配列類似性
Belongs to the P2X receptor family. -
翻訳後修飾
Phosphorylation results in its inactivation.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 18439 Mouse
- Entrez Gene: 29665 Rat
- SwissProt: Q9Z1M0 Mouse
- SwissProt: Q64663 Rat
- Unigene: 42026 Mouse
- Unigene: 10510 Rat
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別名
- ATP receptor antibody
- P2rx7 antibody
- P2RX7_HUMAN antibody
see all
画像
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Western blot - Anti-P2X7 antibody (ab109054)Anti-P2X7 antibody (ab109054) at 1 µg/ml + Rat Hippocampus Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 20 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (ab109054)IHC image of ab109054 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109054, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab109054 は 9 報の論文で使用されています。
- Zhu Y et al. P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy. Exp Ther Med 23:161 (2022). PubMed: 35069842
- Liu L et al. Epac1 and PKA agonists inhibit ROS to reduce NLRP3 inflammasome proteins in retinal endothelial cells. Mol Vis 28:500-506 (2022). PubMed: 37089701
- Li Z et al. Moderate-intensity exercise alleviates pyroptosis by promoting autophagy in osteoarthritis via the P2X7/AMPK/mTOR axis. Cell Death Discov 7:346 (2021). PubMed: 34759265
- Cheng W et al. Paraventricular Nucleus P2X7 Receptors Aggravate Acute Myocardial Infarction Injury via ROS-Induced Vasopressin-V1b Activation in Rats. Neurosci Bull 37:641-656 (2021). PubMed: 33620697
- Wu Y et al. BDNF Participates in Chronic Constriction Injury-Induced Neuropathic Pain via Transcriptionally Activating P2X7 in Primary Sensory Neurons. Mol Neurobiol N/A:N/A (2021). PubMed: 33963520
- Liu Y et al. The P2X7 receptor (P2X7R)-specific antagonist A804598 inhibits inflammatory reaction in human fibroblast-like synoviocytes. Am J Transl Res 12:45-53 (2020). PubMed: 32051736
- Yang J et al. High glucose inhibits osteogenic differentiation and proliferation of MC3T3-E1 cells by regulating P2X7. Mol Med Rep 20:5084-5090 (2019). PubMed: 31702818
- Zhou J et al. Up-regulation of P2X7 Receptors Contributes to Spinal Microglial Activation and the Development of Pain Induced by BmK-I. Neurosci Bull N/A:N/A (2019). PubMed: 30820829
- Zhou J et al. Involvement of P2X7 receptors in satellite glial cells of dorsal root ganglia in the BmK I -induced pain model of rats. Gen Physiol Biophys 38:407-416 (2019). PubMed: 31595882