Anti-P2X4 抗体 (ab168939)
Key features and details
- Mouse polyclonal to P2X4
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-P2X4 antibody
P2X4 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to P2X4 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant full length protein within Human P2X4 aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_002551.2 -
ポジティブ･コントロール
- P2X4 transfected 293T cell lysate; P2X4 expressing 293 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168939の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
|
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
ターゲット情報
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機能
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. -
配列類似性
Belongs to the P2X receptor family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 338036 Cow
- Entrez Gene: 5025 Human
- Entrez Gene: 18438 Mouse
- Entrez Gene: 29659 Rat
- Omim: 600846 Human
- SwissProt: Q5E9U1 Cow
- SwissProt: Q99571 Human
- SwissProt: Q9JJX6 Mouse
see all -
別名
- AI504491 antibody
- ATP gated cation channel protein antibody
- ATP receptor antibody
see all
画像
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Flow Cytometry - Anti-P2X4 antibody (ab168939)
Flow cytometric analysis of negative control 293 cells (Black) and P2X4 expressing 293 cells (Green) labeling P2X4 with ab168939 at 1 ug/ml.
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Western blot - Anti-P2X4 antibody (ab168939)All lanes : Anti-P2X4 antibody (ab168939) at 1 µg/ml
Lane 1 : P2X4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
データシートおよび資料
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Datasheet download
参考文献 (2)
ab168939 は 2 報の論文で使用されています。
- Dave KM et al. Characterization of the SIM-A9 cell line as a model of activated microglia in the context of neuropathic pain. PLoS One 15:e0231597 (2020). PubMed: 32287325
- Chatterjee C & Sparks DL P2X receptors regulate adenosine diphosphate release from hepatic cells. Purinergic Signal 10:587-93 (2014). PubMed: 25059924
