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リコンビナントRabMAb

Anti-P2X3 抗体 [EPR26568-3] (ab300493)

Submit a review Submit a question References (2)
Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
  • Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
  • Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)
  • Immunohistochemistry (Frozen sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)
  • Immunohistochemistry (Frozen sections) - Rabbit monoclonal [EPR26568-3] to P2X3 (AB300493)
  • Immunoprecipitation - Anti-P2X3 antibody [EPR26568-3] (AB300493)
  • Anti-P2X3 antibody [EPR26568-3] (AB300493)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26568-3] to P2X3
  • Suitable for: IHC-Fr, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-P2X3 antibody [EPR26568-3]
    P2X3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26568-3] to P2X3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, IP, IHC-P, WBmore details
    適用なし: Flow Cyt (Intra) or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat and mouse dorsal ganglion tissue lysate. Human heart tissue lysate. IHC-P: Human, mouse and rat dorsal root ganglion tissue. IHC-FR: Rat and mouse dorsal root ganglion tissue (fresh). IP: Rat dorsal ganglion tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300493の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/50.

Not Applicable.
IP
1/30.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 44 kDa.
特記事項
IHC-Fr
1/50.

Not Applicable.
IP
1/30.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 44 kDa.
追加情報
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

ターゲット情報

画像

  • Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Anti-P2X3 antibody [EPR26568-3] (ab300493) at 1/1000 dilution + Human heart tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 44 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking / Diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Anti-P2X3 antibody [EPR26568-3] (ab300493) at 1/1000 dilution + Mouse dorsal ganglion tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking / Diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Western blot - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    All lanes : Anti-P2X3 antibody [EPR26568-3] (ab300493) at 1/1000 dilution

    Lane 1 : Rat dorsal ganglion tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking / Diluting buffer: 5% NFDM/TBST.

    Negative control: Rat heart (PMID: 7566119).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion labeling P2X3 with ab300493 at 1/5000 dilution (0.1 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. The section was incubated with ab300493 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

    Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglion labeling P2X3 with ab300493 at 1/5000 dilution (0.1 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. The section was incubated with ab300493 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

    Immunohistochemical analysis of paraffin-embedded human dorsal root ganglion labeling P2X3 with ab300493 at 1/2000 dilution (0.249 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. The section was incubated with ab300493 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Immunohistochemistry (Frozen sections) - Anti-P2X3 antibody [EPR26568-3] (AB300493)

    Immunohistochemical analysis of frozen mouse dorsal root ganglion labeling P2X3 with ab300493 at 1/50 dilution (9.96 μg/ml). Fresh mouse dorsal root ganglion was fixed with 4% PFA and permeabilized with 0.2 % Triton X-100. ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was used as a secondary antibody at 1/1000 (2 μg/mL) (Green). DAPI was used as a nuclear counterstain (Blue). Positive cytoplasmic staining in mouse dorsal root ganglion.

  • Immunohistochemistry (Frozen sections) - Rabbit monoclonal [EPR26568-3] to P2X3 (AB300493)
    Immunohistochemistry (Frozen sections) - Rabbit monoclonal [EPR26568-3] to P2X3 (AB300493)

    Immunohistochemical analysis of frozen rat dorsal root ganglion labeling P2X3 with ab300493 at 1/50 dilution (9.96 μg/ml). Fresh rat dorsal root ganglion was fixed with 4% PFA and permeabilized with 0.2 % Triton X-100. ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was used as a secondary antibody at 1/1000 (2 μg/mL) (Green). DAPI was used as a nuclear counterstain (Blue). Positive cytoplasmic staining in rat dorsal root ganglion.

  • Immunoprecipitation - Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Immunoprecipitation - Anti-P2X3 antibody [EPR26568-3] (AB300493)

    P2X3 was immunoprecipitated from 10 μg rat dorsal ganglion tissue lysate with ab300493 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab300493 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.


    Lane 1: Rat dorsal ganglion tissue lysate 10 μg (Input).
    Lane 2: ab300493 IP rat dorsal ganglion tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300493 in rat dorsal ganglion tissue lysate.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Observed MW(KDa): 60.

  • Anti-P2X3 antibody [EPR26568-3] (AB300493)
    Anti-P2X3 antibody [EPR26568-3] (AB300493)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab300493 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300493 は 2 報の論文で使用されています。

  • Luo Z  et al. Polyphyllin VI screened from Chonglou by cell membrane immobilized chromatography relieves inflammatory pain by inhibiting inflammation and normalizing the expression of P2X3 purinoceptor. Front Pharmacol 14:1117762 (2023). PubMed: 36865911
  • Kc E  et al. GFAP-NpHR mediated optogenetic inhibition of trigeminal nucleus caudalis attenuates hypersensitive behaviors and thalamic discharge attributed to infraorbital nerve constriction injury. J Headache Pain 24:137 (2023). PubMed: 37821818

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