Anti-p114RhoGEF 抗体 (ab10152)
Key features and details
- Goat polyclonal to p114RhoGEF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-p114RhoGEF antibody
p114RhoGEF 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to p114RhoGEF -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide: SAKEDASKEDVIFF, corresponding to C terminal amino acids 1037-1050 of Human p114RhoGEF.
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特記事項
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10152の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. 1 hour primary incubation is recommended for this product. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 23370 Human
- SwissProt: Q6ZSZ5 Human
- Unigene: 465761 Human
- Unigene: 736818 Human
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別名
- 114 kDa Rho specific guanine nucleotide exchange factor antibody
- 114 kDa Rho-specific guanine nucleotide exchange factor antibody
- ARHGEF 18 antibody
see all
画像
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Western blot - Anti-p114RhoGEF antibody (ab10152)All lanes : Anti-p114RhoGEF antibody (ab10152) at 0.5 µg/ml
Lane 1 : Daudi cell lysate (protein in RIPA buffer)
Lane 2 : Caco-2 cell lysate (protein in RIPA buffer)
Lysates/proteins at 35 µg per lane.Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab10152 は 2 報の論文で使用されています。
- Acharya BR et al. A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. Dev Cell 47:439-452.e6 (2018). PubMed: 30318244
- Tsuji T et al. Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells. Mol Biol Cell 21:3590-600 (2010). PubMed: 20810787