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リコンビナントRabMAb

Anti-P cadherin 抗体 [EPR22426] - BSA and Azide free (ab245204)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
  • Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
  • Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
  • Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22426] to P cadherin - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-P cadherin antibody [EPR22426] - BSA and Azide free
    P cadherin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22426] to P cadherin - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human breast and breast cancer tissues. ICC/IF: A431 and BxPC-3 cells.

  • 特記事項

    ab245204 is the carrier-free version of ab242060.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245204の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

  • 組織特異性

    Expressed in some normal epithelial tissues and in some carcinoma cell lines.

  • 関連疾患

    Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
    Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.

  • 配列類似性

    Contains 5 cadherin domains.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CADH3_HUMAN antibody
      • Cadherin 3 antibody
      • Cadherin 3 precursor antibody
      • Cadherin 3 type 1 antibody
      • Cadherin-3 antibody
      • Cadp antibody
      • Calcium dependent adhesion protein placental antibody
      • CDH 3 antibody
      • CDH3 antibody
      • CDH3 protein antibody
      • CDHP antibody
      • HJMD antibody
      • P cadherin (placental) antibody
      • P-cadherin antibody
      • PCAD antibody
      • Placental cadherin antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)

      Immunohistochemical analysis of paraffin-embedded human breast tissue labeling P cadherin with ab242060 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in ductal epithelium of human breast (PMID: 25424750, 2702654) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242060).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
      Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling P cadherin with ab242060 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in BxPC-3 cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242060).
    • Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
      Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling P cadherin with ab242060 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in A431 cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242060).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling P cadherin with ab242060 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in human breast cancer (PMID: 28084338) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242060).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)
      Anti-P cadherin antibody [EPR22426] - BSA and Azide free (ab245204)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245204 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245204 は論文での使用が確認できていません。

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