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リコンビナントRabMAb

Anti-Oxytocin Receptor 抗体 [EPR24409-146]- BSA and Azide free (ab300444)

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Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (ab300444)
  • Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (ab300444)
  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
  • Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24409-146] to Oxytocin Receptor
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free
    Oxytocin Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24409-146] to Oxytocin Receptor

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat uterus, Rat testis, Mouse uterus, Mouse testis, Human testis, U-2 OS, Untreated C2C12 and C2C12 differentiation for 7 days, whole lysates. ICC: U-2 OS and Rat-1 cells.

  • 特記事項

    ab300444 is a carrier free version of ab300443.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300444の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.

ターゲット情報

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (ab300444)
    Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (ab300444)

    This data was developed using ab300443, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat-1 (rat embryonic fibroblast) cells lebelling Oxytocin Receptor with ab300443 at 1/50 dilution (9.8 µg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Rat-1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS was used instead of primary antibody follwed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (ab300444)
    Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (ab300444)

    This data was developed using ab300443, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells lebelling Oxytocin Receptor with ab300443 at 1/50 dilution (9.8 µg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-2 OS cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS was used instead of primary antibody follwed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
    Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
    All lanes : Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate 20 µg
    Lane 2 : Untreated C2C12 (mouse myoblasts myoblast), whole cell lysate
    Lane 3 : C2C12 differentiation for 7 days, whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution


    This data was developed using 300443, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28382233).

     

    The blot of lane 1 was developed using a high sensitivity ECL substrate.

    3 minutes

    Exposure time: 

  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
    Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
    All lanes : Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate at 20 µg
    Lane 2 : Human skeletal muscle tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution


    This data was developed using 300443, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11467983).

    Negative control: skeletal muscle  (PMID: 11467983).

    Exposure time: 3 minutes

     

  • Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
    Western blot - Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
    All lanes : Anti-Oxytocin Receptor antibody [EPR24409-146] (ab300443) at 1/1000 dilution

    Lane 1 : Rat uterus tissue lysate 20 µg
    Lane 2 : Rat skeletal muscle tissue lysate
    Lane 3 : Rat testis tissue lysate
    Lane 4 : Mouse uterus tissue lysate
    Lane 5 : Mouse skeletal muscle tissue lysate
    Lane 6 : Mouse testis tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution


    This data was developed using 300443, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11467983).

    Negative control: skeletal muscle  (PMID: 11467983).

    Exposure time: 3 minutes

     

  • Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)
    Anti-Oxytocin Receptor antibody [EPR24409-146]- BSA and Azide free (AB300444)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300444 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300444 は論文での使用が確認できていません。

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