Anti-OVCA1 抗体 [EPR15342] - C-terminal (ab185960)
![Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)](https://www.abcam.co.jp/ps/products/185/ab185960/Images/ab185960-219354-anti-ovca1-antibody-epr15342-c-terminal-immunoprecipitation.jpg "Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15342] to OVCA1 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-OVCA1 antibody [EPR15342] - C-terminal
OVCA1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15342] to OVCA1 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- SK OV 3, HeLa, SKBR3 and A375 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15342 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185960の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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| IP |
1/30 - 1/50.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
|
IP
1/30 - 1/50. |
ターゲット情報
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機能
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival. -
組織特異性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. Reduced expression in primary breast and ovarian tumors. -
配列類似性
Belongs to the DPH1/DPH2 family. DPH1 subfamily. -
細胞内局在
Nucleus. Cytoplasm. Punctate, primarily perinuclear localization. - Information by UniProt
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参照データベース
- Entrez Gene: 1801 Human
- Omim: 603527 Human
- SwissProt: Q9BZG8 Human
- Unigene: 513856 Human
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別名
- Candidate tumor suppressor in ovarian cancer 1 antibody
- Diphthamide biosynthesis protein 1 antibody
- Diphthamide biosynthesis protein 2 homolog like 1 antibody
see all
画像
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Immunoprecipitation - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)
Western blot analysis of immunoprecipitation pellet from SKBR3 cell lysate immunoprecipitated using ab185960 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)](https://www.abcam.co.jp/ps/products/185/ab185960/Images/ab185960-219352-anti-ovca1-antibody-epr15342-c-terminal-western-blot.jpg)
Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)All lanes : Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960) at 1/10000 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : A375 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 49 kDa -
![Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)](https://www.abcam.co.jp/ps/products/185/ab185960/Images/ab185960-219351-anti-ovca1-antibody-epr15342-c-terminal-western-blot.jpg)
Western blot - Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)All lanes : Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960) at 1/1000 dilution
Lane 1 : SK OV 3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 49 kDa -
![Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)](https://www.abcam.co.jp/ps/products/185/ab185960/Images/ab185960-4-benefits-of-recombinant-antibodies.png)
Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab185960 は 2 報の論文で使用されています。
- Togami K et al. DNA methyltransferase inhibition overcomes diphthamide pathway deficiencies underlying CD123-targeted treatment resistance. J Clin Invest 129:5005-5019 (2019). PubMed: 31437130
- Liu M et al. Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p. Cell Physiol Biochem 44:505-514 (2017). PubMed: 29145210