AB254267

Anti-OTP 抗体 [EPR22178-17]

Anti-OTP antibody [EPR22178-17]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal OTP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

別名を表示する

Homeobox protein orthopedia, OTP

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling OTP with ab254267 at 1/4000 dilution (0.113μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control : No staining in human lung squamous cell carcinoma (PMID : 26927888) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemical analysis of paraffin-embedded human lung carcinoid tissue labeling OTP with ab254267 at 1/4000 dilution (0.113μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human lung carcinoid (PMID : 30284410, 29316326) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemical analysis of paraffin-embedded human lung small cell carcinoma tissue labeling OTP with ab254267 at 1/4000 dilution (0.113μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human lung small cell carcinoma (PMID : 26927888) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling OTP with ab254267 at 1/4000 dilution (0.113μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control : No staining in human lung (PMID : 26927888) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22178-17

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

OTP also known as orthopedia homeobox is a transcription factor involved in the development and differentiation of neuroendocrine cells. It belongs to the homeobox family of proteins which are characterized by a specific DNA-binding domain. OTP typically weighs around 35 kDa. This protein is mainly expressed in the central nervous system and neuroendocrine tissues particularly within the hypothalamus.

Biological function summary

OTP plays an important role in the regulation of gene expression during the development of specific neuronal cell types. It is not part of a larger complex but interacts with various other transcription factors to influence cell fate decisions. OTP's regulatory actions are essential during embryonic development where it directs the differentiation and specialization of neurons.

Pathways

OTP is significant in the development pathways of the nervous system. It participates in neurogenesis particularly influencing the hypothalamic lineage. OTP interacts with proteins such as Nkx2.1 and Sim1 which are also transcription factors that guide the specification of neuroendocrine neurons. These interactions highlight OTP’s involvement in complex genetic regulatory networks essential for proper nervous system function.

OTP shows relevance to certain neurodevelopmental disorders and tumors. Abnormal OTP expression links to the development of medullary thyroid carcinoma a type of cancer affecting thyroid gland cells. Furthermore OTP's expression patterns can aid in distinguishing certain tumor types in neuropathology. Researchers study its interaction with proteins like RET which is also implicated in thyroid cancer to better understand and treat these diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Probably involved in the differentiation of hypothalamic neuroendocrine cells.

See full target information OTP

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:5618-5633 PubMed38499392

2024

Predicting the prognosis of glioma patients with TERT promoter mutations and guiding the specific immune profile of immune checkpoint blockade therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenpeng Cao,Jinzhi Lan,Chujiao Hu,Jinping Kong,Limin Xiang,Zhixue Zhang,Yating Sun,Zhirui Zeng,Shan Lei

Archives of pathology & laboratory medicine 147:1446-1450 PubMed36800547

2023

Molecular Characterization of Testicular Mesothelioma and the Role of Asbestos as a Causative Factor.

Applications

Unspecified application

Species

Unspecified reactive species

Ashleigh Jean Hocking,Elaine May Thomas,Sarita Prabhakaran,Alexandra Jolley,Susan Lesley Woods,Matthew J Soeberg,Sonja Klebe

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OTP 抗体 [EPR22178-17]

Anti-OTP antibody [EPR22178-17]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise