AB216406

Anti-Osteopontin 抗体

Name: Anti-Osteopontin 抗体 (ab216406) | Abcam
Brand: Abcam Limited
SKU: AB216406
Price: 75000 JPY
Availability: InStock
Rating: 4 (2 reviews)

Anti-Osteopontin antibody

(2 Reviews)

(3 Publications)

Rabbit Polyclonal Osteopontin antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SPP1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216406)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216406)

Immunohistochemical analysis of formalin fixed paraffin-embedded human stomach tissue sections labeling Osteopontin with ab216406 at a 1/200 dilution. DAB staining.

Supplier Data

Western blot - Anti-Osteopontin antibody (AB216406)

All lanes:

Western blot - Anti-Osteopontin antibody (ab216406) at 1/300 dilution

All lanes:

mouse kidney lysates

Predicted band size: 35 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human SPP1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10451

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代替製品

Primary Antibodies

AB8448

Anti-Osteopontin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB8448)

Primary Antibodies

AB63856

Anti-Osteopontin antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody (AB63856)

Primary Antibodies

AB218237

Anti-Osteopontin antibody [EPR21138]

RabMAb|Recombinant|20ul selling size|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (AB218237)

Primary Antibodies

AB214050

Anti-Osteopontin antibody [EPR21139-316]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (AB214050)

Primary Antibodies

AB229854

Anti-Osteopontin antibody [EPR21138] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] - BSA and Azide free (AB229854)

Primary Antibodies

AB236213

Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (AB236213)

組合せ製品

Assay Kits

AB269374

Human Osteopontin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Osteopontin ELISA Kit (AB269374)

Primary Antibodies

AB93876

Anti-Osteocalcin antibody

Western blot - Anti-Osteocalcin antibody (AB93876)

Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

Primary Antibodies

AB209484

Anti-Sp7 / Osterix antibody [EPR21034]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] (AB209484)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.

Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

See full target information SPP1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 13:1168772 PubMed37251946

2023

Identification of the hub genes associated with prostate cancer tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Honghui Zhu,Qi Lin,Xiaomin Gao,Xixi Huang

Molecular medicine reports 23: PubMed33179111

2020

Astragaloside‑IV modulates NGF‑induced osteoblast differentiation via the GSK3β/β‑catenin signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan-Yang Sun,Xiao-Lan Liu,Juan Gao,Xiao-Hui Wu,Ben Dou

Scientific reports 7:46679 PubMed28447667

2017

TWEAK blockade decreases atherosclerotic lesion size and progression through suppression of STAT1 signaling in diabetic mice.

Applications

IHC

Species

Mouse

Valvanera Fernández-Laso,Cristina Sastre,Nerea Méndez-Barbero,Jesús Egido,Jose L Martín-Ventura,Carmen Gómez-Guerrero,Luis M Blanco-Colio

View all publications

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Abcam product promise

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