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リコンビナントRabMAb

Anti-OSBPL9 抗体 [EPR9333] (ab151691)

Reviews (1) Submit a question References (1)
Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (ab151691)
  • Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (ab151691)
  • Anti-OSBPL9 antibody [EPR9333] (ab151691)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9333] to OSBPL9
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-OSBPL9 antibody [EPR9333]
    OSBPL9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9333] to OSBPL9

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human OSBPL9 aa 650-750. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • Human fetal brain, Jurkat, 293T and HepG2 cell lysates; Human kidney tissue; 293T cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151691の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    The OSBPL9 gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. OSBPL9 appears to be in a distinct OSBP subfamily and shares relatively little homology in the sterol-binding domain with other OSBPs. Multiple transcript variants have been identified, most of which encode distinct isoforms.

  • 細胞内局在

    Golgi Apparatus.

  • 参照データベース

    • 別名

      • FLJ12492 antibody
      • FLJ14629 antibody
      • FLJ14801 antibody
      • FLJ32055 antibody
      • FLJ34384 antibody
      • MGC15035 antibody
      • ORP9 antibody
      • OSBP related protein 9 antibody
      • OSBP4 antibody
      • Oxysterol binding protein like 9 antibody
      • Oxysterol binding protein related protein 9 antibody
      • oxysterol binding protein-like 9 antibody
      • oxysterol binding proteinlike9 antibody
      see all

    画像

    • Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)
      Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691)
      All lanes : Anti-OSBPL9 antibody [EPR9333] (ab151691) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 83 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (ab151691)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (ab151691)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling OSBPL9 with ab151691 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (ab151691)
      Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (ab151691)

      Intracellular flow cytometric analysis of permeabilized 293T cells labeling OSBPL9 with ab151691 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

       

    • Anti-OSBPL9 antibody [EPR9333] (ab151691)
      Anti-OSBPL9 antibody [EPR9333] (ab151691)

    参考文献 (1)

    ab151691 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab151691 は 1 報の論文で使用されています。

    • Gillingham AK  et al. In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Elife 8:N/A (2019). PubMed: 31294692

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-OSBPL9 antibody [EPR9333]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    HEK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 15 2019

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