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AB109510

Anti-OS9 抗体 [EPR4272(2)]

Anti-OS9 antibody [EPR4272(2)]

5

(1 Review)

|

(14 Publications)

Rabbit Recombinant Monoclonal OS9 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 14 publications.

別名を表示する

Protein OS-9, Amplified in osteosarcoma 9, OS9

2 Images
Western blot - Anti-OS9 antibody [EPR4272(2)] (AB109510)
  • WB

Unknown

Western blot - Anti-OS9 antibody [EPR4272(2)] (AB109510)

All lanes:

Western blot - Anti-OS9 antibody [EPR4272(2)] (ab109510) at 1/10000 dilution

Lane 1:

NIH3T3 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HL-60 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 76 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody [EPR4272(2)] (AB109510)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody [EPR4272(2)] (AB109510)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-OS9 antibody [EPR4272(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4272(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

OS9 also known as osteosarcoma amplified 9 protein has a molecular mass of approximately 84 kDa. The protein primarily locates in the endoplasmic reticulum (ER). OS9 functions as an ER-associated protein that plays an important role in the recognition and targeting of misfolded glycoproteins for degradation through the ER-associated degradation (ERAD) pathway. It assembles as part of the ERAD machinery through interactions with glycoproteins via its mannose 6-phosphate receptor homology (MRH) domain directly influencing the fate of proteins that fail proper folding.
Biological function summary

OS9 plays an important role in protein quality control within the cell. It forms part of a complex that detects and binds misfolded glycoproteins therefore marking them for transport to the proteasome for degradation. This function aids in maintaining cellular homeostasis by preventing the accumulation of defective proteins that can lead to cellular stress and dysfunction. The protein works in coordination with other ER chaperones and components such as SEL1L and HERPUD1 facilitating efficient substrate delivery to the degradation pathway.

Pathways

OS9 integrates into the ERAD pathway which is important for managing the protein folding load within the ER. This process is essential in managing and mitigating ER stress linked to the unfolded protein response (UPR). In particular OS9 interacts with ER mannosyl-oligosaccharide 12-alpha-mannosidase (MAN1B1) to trim mannose a vital step in determining protein fate within this pathway. Additionally OS9 links with proteins such as XBP1 helping regulate cellular stress responses.

OS9 has implications in neurodegenerative diseases and cancer. Misregulation or mutations affecting its function can contribute to diseases characterized by protein misfolding such as Alzheimer's disease where it might interact with other proteins involved in amyloid precursor protein (APP) processing. Additionally in the context of cancer overexpression correlates with tumor progression in certain cancers as it may influence pathways involving HERPUD1 affecting cell survival and proliferation under stress conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
See full target information Protein OS-9
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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed37943617

2024

Biallelic Cys141Tyr variant of SEL1L is associated with neurodevelopmental disorders, agammaglobulinemia, and premature death.

Applications

Unspecified application

Species

Unspecified reactive species

Denisa Weis,Liangguang L Lin,Huilun H Wang,Zexin Jason Li,Katarina Kusikova,Peter Ciznar,Hermann M Wolf,Alexander Leiss-Piller,Zhihong Wang,Xiaoqiong Wei,Serge Weis,Katarina Skalicka,Gabriela Hrckova,Lubos Danisovic,Andrea Soltysova,Tingxuan T Yang,René Günther Feichtinger,Johannes A Mayr,Ling Qi

The Journal of clinical investigation 134: PubMed37943610

2024

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Huilun H Wang,Liangguang L Lin,Zexin J Li,Xiaoqiong Wei,Omar Askander,Gerarda Cappuccio,Mais O Hashem,Laurence Hubert,Arnold Munnich,Mashael Alqahtani,Qi Pang,Margit Burmeister,You Lu,Karine Poirier,Claude Besmond,Shengyi Sun,Nicola Brunetti-Pierri,Fowzan S Alkuraya,Ling Qi

JCI insight 8: PubMed37345654

2023

Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid gland.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohan Zhang,Crystal Young,Xiao-Hui Liao,Samuel Refetoff,Mauricio Torres,Yaron Tomer,Mihaela Stefan-Lifshitz,Hao Zhang,Dennis Larkin,Deyu Fang,Ling Qi,Peter Arvan

Nature communications 14:3132 PubMed37253728

2023

The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangcheng Alivia Wu,Chenchen Shen,Xiaoqiong Wei,Xiawei Zhang,Siwen Wang,Xinxin Chen,Mauricio Torres,You Lu,Liangguang Leo Lin,Huilun Helen Wang,Allen H Hunter,Deyu Fang,Shengyi Sun,Magdalena I Ivanova,Yi Lin,Ling Qi

Nature cell biology 25:726-739 PubMed37142791

2023

SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING pool.

Applications

Unspecified application

Species

Unspecified reactive species

Yewei Ji,Yuan Luo,Yating Wu,Yao Sun,Lianfeng Zhao,Zhen Xue,Mengqi Sun,Xiaoqiong Wei,Zinan He,Shuangcheng Alivia Wu,Liangguang Leo Lin,You Lu,Lei Chang,Fei Chen,Siyu Chen,Wei Qian,Xiaoxi Xu,Shengnuo Chen,Dongli Pan,Zhangsen Zhou,Sheng Xia,Chih-Chi Andrew Hu,Tingbo Liang,Ling Qi

Proceedings of the National Academy of Sciences of the United States of America 120:e2212644120 PubMed36595688

2023

Hepatic SEL1L-HRD1 ER-associated degradation regulates systemic iron homeostasis via ceruloplasmin.

Applications

Unspecified application

Species

Unspecified reactive species

Pattaraporn Thepsuwan,Asmita Bhattacharya,Zhenfeng Song,Stephen Hippleheuser,Shaobin Feng,Xiaoqiong Wei,Nupur K Das,Mariana Sierra,Juncheng Wei,Deyu Fang,Yu-Ming M Huang,Kezhong Zhang,Yatrik M Shah,Shengyi Sun

Gut microbes 14:2136467 PubMed36261888

2022

299v supplementation modulates β-cell ER stress and antioxidative defense pathways and prevents type 1 diabetes in gluten-free BioBreeding rats.

Applications

Unspecified application

Species

Unspecified reactive species

Pinar Sargin,Mark F Roethle,Shuang Jia,Tarun Pant,Ashley E Ciecko,Samantha N Atkinson,Nita H Salzman,Ru-Jeng Teng,Yi-Guang Chen,Susanne M Cabrera,Martin J Hessner

Molecular biology of the cell 33:ar120 PubMed36074076

2022

A small-molecule inhibitor and degrader of the RNF5 ubiquitin ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Ruan,Dongdong Liang,Wenjing Yan,Yongwang Zhong,Daniel C Talley,Ganesha Rai,Dingyin Tao,Christopher A LeClair,Anton Simeonov,Yinghua Zhang,Feihu Chen,Nancy L Quinney,Susan E Boyles,Deborah M Cholon,Martina Gentzsch,Mark J Henderson,Fengtian Xue,Shengyun Fang

Cell chemical biology 28:46-59.e7 PubMed32888501

2020

Proteostasis Regulators Restore Function of Epilepsy-Associated GABA Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jing Di,Ya-Juan Wang,Edmund Cotter,Meng Wang,Angela L Whittsette,Dong-Yun Han,Panjamaporn Sangwung,Renae Brown,Joseph W Lynch,Angelo Keramidas,Ting-Wei Mu

eLife 9: PubMed32720893

2020

Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Galeone,Joshua M Adams,Shinya Matsuda,Maximiliano F Presa,Ashutosh Pandey,Seung Yeop Han,Yuriko Tachida,Hiroto Hirayama,Thomas Vaccari,Tadashi Suzuki,Cathleen M Lutz,Markus Affolter,Aamir Zuberi,Hamed Jafar-Nejad
View all publications
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保証に関する詳細については利用規約をご確認ください。
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関連製品

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