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AB262865

Anti-Ornithine Carbamoyltransferase/OTC 抗体 [CL4046]

Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046]

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Mouse Monoclonal Ornithine Carbamoyltransferase/OTC antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300.

別名を表示する

OTCase, OTC

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

Paraffin-embedded human small intestine tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

Paraffin-embedded human endometrium tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis. No expression, as expected.

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for Ornithine Carbamoyltransferase/OTC (green) using ab262865 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

Paraffin-embedded human liver tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (AB262865)

Paraffin-embedded human duodenum tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CL4046

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

P00480

エピトープ

Binds to an epitope located within the peptide sequence GYEPDASVTKLAEQY as determined by overlapping synthetic peptides.

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代替製品

Primary Antibodies

AB262864

Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4045]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4045] (AB262864)

  • 0
  • 1
Primary Antibodies

AB262866

Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4049]

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4049] (AB262866)

  • 0
  • 0
Primary Antibodies

AB222399

Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (AB222399)

  • 0
  • 0
Primary Antibodies

AB180401

Anti-Ornithine Carbamoyltransferase/OTC antibody [OTI8A1]

  • 0
  • 0
Primary Antibodies

AB244243

Anti-Ornithine Carbamoyltransferase/OTC antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody (AB244243)

  • 0
  • 0
Primary Antibodies

AB203859

Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

  • 5
  • 1

組合せ製品

Assay Kits

AB252903

Ornithine Assay Kit (Fluorometric)

Functional Studies - Ornithine Assay Kit (Fluorometric) (AB252903)

  • 0
  • 0
Primary Antibodies

AB170952

Anti-ASS1 antibody [EPR12398]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (AB170952)

  • 5
  • 1
Proteins & Peptides

AB123452

Recombinant Human Ornithine Carbamoyltransferase/OTC protein

Western blot - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (AB123452)

  • 0
  • 0
Primary Antibodies

AB97370

Anti-Argininosuccinate Lyase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (AB97370)

  • 4
  • 1
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine Carbamoyltransferase (OTC) also known as ornithine aminotransferase is an enzyme critical in the urea cycle. The OTC protein facilitates the conversion of ornithine and carbamoyl phosphate into citrulline a process essential for nitrogen excretion. The human OTC protein has a mass of approximately 36 kilodaltons and is primarily expressed in the liver mitochondria. Protein assays and procurement efforts often focus on obtaining accurate measurements and ensuring high-purity OTC peptides enabling proper functional studies.
Biological function summary

Ornithine carbamoyltransferase plays a significant role in detoxifying ammonia by forming citrulline an important urea cycle intermediate. The protein functions as a homotrimer meaning three identical subunits form an active enzyme complex. This multimeric state facilitates efficient substrate processing allowing effective handling of nitrogen waste. The integral role of OTC in the urea cycle highlights its importance in maintaining systemic nitrogen balance.

Pathways

OTC is a central component of the urea cycle and influences nitrogen metabolism pathways. These pathways include the conversion of ammonia to urea an important detoxifying process for safe excretion by the kidneys. OTC closely interacts with enzymes such as argininosuccinate synthetase highlighting the coordinated action required to advance urea formation. Understanding OTC's participation within these pathways helps identify its regulatory points and the potential impact of genetic variations.

Mutations in the OTC gene can lead to ornithine transcarbamylase deficiency a disorder affecting ammonia detoxification. This X-linked genetic condition disrupts normal nitrogen metabolism causing ammonia accumulation particularly harmful to the central nervous system. OTC deficiency shares a functional relationship with other urea cycle disorders such as citrullinemia due to its contribution to the same biochemical pathway. Researchers focus on identifying OTC protein mutations to improve diagnosis and treatment of these conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline (PubMed : 2556444, PubMed : 6372096, PubMed : 8112735). The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion (PubMed : 2556444).
See full target information Ornithine transcarbamylase, mitochondrial
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