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AB9641

Anti-ORC4L 抗体

Anti-ORC4L antibody

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(11 Publications)

Goat Polyclonal ORC4L antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human ORC4 aa 400 to C-terminus.

別名を表示する

ORC4L, ORC4, Origin recognition complex subunit 4

4 Images
Western blot - Anti-ORC4L antibody (AB9641)
  • WB

Unknown

Western blot - Anti-ORC4L antibody (AB9641)

ab9641 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-ORC4L antibody (ab9641)

Predicted band size: 50 kDa

false

Western blot - Anti-ORC4L antibody (AB9641)
  • WB

Unknown

Western blot - Anti-ORC4L antibody (AB9641)

All lanes:

Western blot - Anti-ORC4L antibody (ab9641) at 0.5 µg/mL

All lanes:

Rat Heart lysate in RIPA buffer at 35 µg

Predicted band size: 50 kDa

Observed band size: 50 kDa

true

Western blot - Anti-ORC4L antibody (AB9641)
  • WB

Unknown

Western blot - Anti-ORC4L antibody (AB9641)

All lanes:

Western blot - Anti-ORC4L antibody (ab9641) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 50 kDa

Observed band size: 38 kDa,49 kDa

true

Exposure time: 20min

Western blot - Anti-ORC4L antibody (AB9641)
  • WB

Supplier Data

Western blot - Anti-ORC4L antibody (AB9641)

All lanes:

Western blot - Anti-ORC4L antibody (ab9641) at 0.5 µg/mL

All lanes:

Mouse heart lysate at 35 µg

Predicted band size: 50 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human ORC4 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43929

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The origin recognition complex subunit 4-like (ORC4L) also known simply as ORC4 is a mechanically important player in the initiation of DNA replication. ORC4 typically has a molecular mass of approximately 50 kDa. It is highly expressed in cells that are actively dividing especially within the nucleus where replication origins are located. The protein forms part of the larger origin recognition complex (ORC) which is important for marking the starting points for DNA replication across eukaryotic organisms.
Biological function summary

ORC4 plays a central role in recognizing replication origins and acts as an anchor for the assembly of other necessary replication proteins. It is one of six subunits that comprise the ORC complex. This protein complex binds to origins of replication in DNA unwinds the double helix and recruits additional factors required for the formation of the pre-replicative complex. The presence of ORC4 is essential in ensuring that DNA replication occurs once and only once per cell cycle preventing genomic instability.

Pathways

Several cellular processes depend on the correct functioning of ORC4. In cell cycle regulation ORC4 is integral to the DNA replication checkpoint which ensures DNA is not damaged before it is duplicated. ORC4 interacts with other replication factors like CDC6 and MCM2-7 helicase to initiate and regulate the replication process. Its activity influences key pathways such as the p53-dependent DNA damage response where ORC4 can affect decisions related to cell cycle arrest or apoptosis.

ORC4 dysregulation links to genomic instability which is a hallmark in the development of cancer. Altered levels or mutations in ORC4 contribute to an increased susceptibility to oncogenesis with links to various cancers such as those of the breast and colon. The BRCA1 protein known for its role in maintaining genomic stability and DNA repair often shows interactions with ORC4 during such disease states highlighting their interconnected roles in cancer-related pathways.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
See full target information ORC4
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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 81:1951-1969.e6 PubMed33761311

2021

Multiple, short protein binding motifs in ORC1 and CDC6 control the initiation of DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Manzar Hossain,Kuhulika Bhalla,Bruce Stillman

Cell cycle (Georgetown, Tex.) 18:605-620 PubMed30741601

2019

Human NOC3 is essential for DNA replication licensing in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Man-Hei Cheung,Aftab Amin,Rentian Wu,Yan Qin,Lan Zou,Zhiling Yu,Chun Liang

Oncotarget 7:80190-80207 PubMed27863397

2016

Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing.

Applications

ICC/IF

Species

Human

Ruowen Zhang,Jiahui Wu,Sylvain Ferrandon,Katie J Glowacki,Janet A Houghton

eLife 5: PubMed27458800

2016

Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription.

Applications

IP

Species

Human

Manzar Hossain,Bruce Stillman

PloS one 10:e0128497 PubMed26038842

2015

Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone.

Applications

WB

Species

Unspecified reactive species

Vladimir V Sherstyuk,Alexander I Shevchenko,Suren M Zakian

The Journal of biological chemistry 287:15024-33 PubMed22427655

2012

Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing.

Applications

WB

Species

Unspecified reactive species

Kui Ming Chan,Zhiguo Zhang

The Journal of biological chemistry 286:23831-41 PubMed21555516

2011

Assembly of the human origin recognition complex occurs through independent nuclear localization of its components.

Applications

WB

Species

Human

Soma Ghosh,Alex P Vassilev,Junmei Zhang,Yingming Zhao,Melvin L DePamphilis

The Journal of biological chemistry 283:2265-74 PubMed18029353

2007

FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.

Applications

WB

Species

Human

Yoshikazu Johmura,Shigehiro Osada,Makoto Nishizuka,Masayoshi Imagawa

The Journal of biological chemistry 281:23264-73 PubMed16762929

2006

Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.

Applications

Unspecified application

Species

Unspecified reactive species

Ilian Radichev,Sung Won Kwon,Yingming Zhao,Melvin L DePamphilis,Alex Vassilev

Molecular cell 19:567-75 PubMed16109380

2005

Human origins of DNA replication selected from a library of nascent DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Vesna Todorovic,Sara Giadrossi,Cristina Pelizon,Ramiro Mendoza-Maldonado,Hisao Masai,Mauro Giacca
View all publications
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