Anti-OPA1 抗体 (ab42364)
Key features and details
- Rabbit polyclonal to OPA1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-OPA1 antibody
OPA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to OPA1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab42364の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 92,86 kDa (predicted molecular weight: 111 kDa).
Abcam recommends using milk as the blocking agent. |
| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92,86 kDa (predicted molecular weight: 111 kDa). Abcam recommends using milk as the blocking agent. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. -
組織特異性
Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. -
関連疾患
Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. -
配列類似性
Belongs to the dynamin family. -
翻訳後修飾
PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. -
細胞内局在
Mitochondrion inner membrane. Mitochondrion intermembrane space. - Information by UniProt
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参照データベース
- Entrez Gene: 424900 Chicken
- Entrez Gene: 4976 Human
- Entrez Gene: 74143 Mouse
- Entrez Gene: 171116 Rat
- Omim: 605290 Human
- SwissProt: O60313 Human
- SwissProt: P58281 Mouse
- SwissProt: Q2TA68 Rat
see all -
別名
- Dynamin like 120 kDa protein antibody
- Dynamin like 120 kDa protein, mitochondrial antibody
- Dynamin-like 120 kDa protein, form S1 antibody
see all
画像
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Western blot - Anti-OPA1 antibody (ab42364)All lanes : Anti-OPA1 antibody (ab42364) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Western blot - Anti-OPA1 antibody (ab42364)Anti-OPA1 antibody (ab42364) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
Additional bands at: 86 kDa (possible isoform)
Although the predicted band size is ~111 kDa based on Swiss-prot data, bands of 92 kDa and 86 kDa has been previously observed. Investigative Ophthalmology & Visual Science, November 2005, Vol. 46, No. 11 -
Immunoprecipitation - Anti-OPA1 antibody (ab42364)OPA1 was immunoprecipitated using 0.5mg Rat Brain whole tissue lysate, 5µg of Rabbit polyclonal to OPA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, rat brain whole tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab42364.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 92kDa: OPA1; non specific - 100kDa: We are unsure as to the identity of this extra band.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (134)
ab42364 は 134 報の論文で使用されています。
- Głombik K et al. The Effects of Prenatal Dexamethasone Exposure on Brain Metabolic Homeostasis in Adulthood: Implications for Depression. Int J Mol Sci 24:N/A (2023). PubMed: 36674678
- Wang Y et al. TIM-4 orchestrates mitochondrial homeostasis to promote lung cancer progression via ANXA2/PI3K/AKT/OPA1 axis. Cell Death Dis 14:141 (2023). PubMed: 36806050
- Koizumi T et al. Empagliflozin suppresses mitochondrial reactive oxygen species generation and mitigates the inducibility of atrial fibrillation in diabetic rats. Front Cardiovasc Med 10:1005408 (2023). PubMed: 36815024
- Yan MQ et al. Mitoguardin2 Is Associated With Hyperandrogenism and Regulates Steroidogenesis in Human Ovarian Granulosa Cells. J Endocr Soc 7:bvad034 (2023). PubMed: 36936714
- Wang Y et al. Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats. Cell Death Dis 14:325 (2023). PubMed: 37179386