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リコンビナントRabMAb

Anti-Olfactory Marker Protein/OMP 抗体 [EPR19190] (ab183947)

Reviews (2) Submit a question References (20)
Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
  • Immunoprecipitation - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
  • Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
  • Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
  • Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19190] to Olfactory Marker Protein/OMP
  • Suitable for: IHC-Fr, IHC-P, WB, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Olfactory Marker Protein/OMP antibody [EPR19190]
    Olfactory Marker Protein/OMP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19190] to Olfactory Marker Protein/OMP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, WB, IPmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse and Rat olfactory bulb lysates. IHC-P: Mouse olfactory bulb tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183947の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/500.
IHC-P (1)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Suitable for use in mouse tissue only.
WB (1)
1/2000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IP
1/40.
特記事項
IHC-Fr
1/500.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Suitable for use in mouse tissue only.
WB
1/2000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IP
1/40.

ターゲット情報

  • 関連性

    Olfactory marker protein (OMP) is an abundant, 19kDa, cytosolic protein that is almost exclusively expressed in mature, functioning, olfactory neurons but not in the neural precursor basal cells. The OMP gene structure and protein sequence are highly conserved between mouse, rat and human. Its tissue specific expression in the receptor cells together with the results of the mouse knockout studies show that OMP represents a novel modulatory component of the odor detection/signal transduction cascade.

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    • 別名

      • Olfactory marker protein antibody
      • Olfactory neuronal specific protein antibody
      • OMP antibody

    画像

    • Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
      Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
      All lanes : Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947) at 1/2000 dilution

      Lane 1 : Mouse olfactory bulb lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Mouse heart lysate
      Lane 4 : Mouse kidney lysate
      Lane 5 : Mouse spleen lysate
      Lane 6 : Neuro-2a (Mouse neuroblastoma cell line) lysate
      Lane 7 : Rat olfactory bulb lysate
      Lane 8 : Rat kidney lysate
      Lane 9 : C6 (Rat glial tumor cell line) lysate
      Lane 10 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) lysate
      Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma cell line) lysate
      Lane 12 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 19 kDa
      Observed band size: 19 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
      Immunoprecipitation - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)

      Olfactory Marker Protein/OMP was immunoprecipitated from 1mg of Mouse olfactory bulb whole cell lysate with ab183947 at 1/40 dilution. Western blot was performed using ab183947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

       

      Lane 1(input): Mouse olfactory bulb whole cell lysate 10μg

      Lane 2: Mouse olfactory bulb whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183947 in Mouse olfactory bulb whole cell lysate

       

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)

      Immunohistochemical analysis of paraffin-embedded Mouse olfactory bulb tissue labeling Olfactory Marker Protein/OMP with ab183947 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on neurons of mouse olfactory bulb is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Olfactory Marker Protein/OMP with ab183947 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative staining on mouse liver is observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
      Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)

      Immunohistochemistry (Frozen sections) analysis of mouse olfactory bulb tissue labelling Olfactory Marker Protein/OMP with ab183947 at a dilution of 1/500. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Staining is visible in the cytoplasm of neurons of the mouse olfactory bulb.

    • Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
      Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)

      Immunohistochemistry (Frozen sections) analysis of rat olfactory bulb tissue labelling Olfactory Marker Protein/OMP with ab183947 at a dilution of 1/500. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Staining is visible in the cytoplasm of neurons of the rat olfactory bulb.

    • Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
      Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)

    参考文献 (20)

    ab183947 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab183947 は 20 報の論文で使用されています。

    • Zhang C  et al. Odor Enrichment Attenuates the Anesthesia/Surgery-induced Cognitive Impairment. Ann Surg 277:e1387-e1396 (2023). PubMed: 35848747
    • Zhou J  et al. Zika virus leads to olfactory disorders in mice by targeting olfactory ensheathing cells. EBioMedicine 89:104457 (2023). PubMed: 36739631
    • Duan Y  et al. Long-Term Flexible Neural Interface for Synchronous Recording of Cross-Regional Sensory Processing along the Olfactory Pathway. Small 19:e2205768 (2023). PubMed: 37035943
    • Lu X  et al. DMBT1 Alleviates Nasal Airway Inflammatory Response in the LPS-Induced Nasal Polyp Model. Int Arch Allergy Immunol 184:808-813 (2023). PubMed: 37232026
    • Fan Y  et al. Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons. Commun Biol 5:411 (2022). PubMed: 35505181
    View all Publications for this product

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Olfactory Marker Protein/OMP antibody [EPR19190]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (olfactory epithelium)
    Gel Running Conditions
    Non-reduced Denaturing (10% and 12%)
    Loading amount
    20 µg
    Specification
    olfactory epithelium
    Blocking step
    BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. yin tzu chen

    Verified customer

    投稿 Aug 26 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Olfactory Marker Protein/OMP antibody [EPR19190]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain and olfactory epithelium)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    brain and olfactory epithelium
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tamar Licht

    Verified customer

    投稿 Feb 10 2020

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