Anti-Olfactory Marker Protein/OMP 抗体 [EPR19157] (ab183948)
![Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)](https://www.abcam.co.jp/ps/products/183/ab183948/Images/ab183948-254103-183948WBa.jpg "Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19157] to Olfactory Marker Protein/OMP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-Olfactory Marker Protein/OMP antibody [EPR19157]
Olfactory Marker Protein/OMP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19157] to Olfactory Marker Protein/OMP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse OMP full length recombinant protein; Mouse and rat olfactory bulb lysates. IHC-P: Mouse and rat olfactory bulb tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19157 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Olfactory marker protein (OMP) is an abundant, 19kDa, cytosolic protein that is almost exclusively expressed in mature, functioning, olfactory neurons but not in the neural precursor basal cells. The OMP gene structure and protein sequence are highly conserved between mouse, rat and human. Its tissue specific expression in the receptor cells together with the results of the mouse knockout studies show that OMP represents a novel modulatory component of the odor detection/signal transduction cascade. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 18378 Mouse
- Entrez Gene: 24612 Rat
- SwissProt: Q64288 Mouse
- SwissProt: P08523 Rat
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別名
- Olfactory marker protein antibody
- Olfactory neuronal specific protein antibody
- OMP antibody
画像
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Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)All lanes : Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948) at 1/1000 dilution
Lane 1 : Rat kidney lysate at 20 µg
Lane 2 : Rat spleen lysate at 20 µg
Lane 3 : Mouse brain lysate at 20 µg
Lane 4 : Mouse heart lysate at 20 µg
Lane 5 : Mouse kidney lysate at 20 µg
Lane 6 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 7 : Mouse OMP full length recombinant protein at 0.02 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
Uniquely associated with mature olfactory receptor neurons.
Mouse OMP full length recombinant protein contains aa 2-163 with a His-Tag®. The recombinant mouse full length protein was made in-house. -
![Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)](https://www.abcam.co.jp/ps/products/183/ab183948/Images/ab183948-254102-wb-2.jpg)
Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)Lane 1 : Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948) at 1/200 dilution
Lane 2 : Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948) at 1/1000 dilution
Lane 1 : Mouse olfactory bulb lysate
Lane 2 : Rat olfactory bulb tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
Uniquely associated with mature olfactory receptor neurons.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)](https://www.abcam.co.jp/ps/products/183/ab183948/Images/ab183948-254101-ihc-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)Immunohistochemical analysis of paraffin-embedded mouse olfactory bulb tissue labeling Olfactory Marker Protein/OMP with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse olfactory bulb is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)](https://www.abcam.co.jp/ps/products/183/ab183948/Images/ab183948-254099-ihc-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Olfactory Marker Protein/OMP with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse liver. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)](https://www.abcam.co.jp/ps/products/183/ab183948/Images/ab183948-254100-ihc-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)Immunohistochemical analysis of paraffin-embedded rat olfactory bulb tissue labeling Olfactory Marker Protein/OMP with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat olfactory bulb is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)](https://www.abcam.co.jp/ps/products/183/ab183948/Images/ab183948-254098-ihc-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)Immunohistochemical analysis of paraffin-embedded rat muscle tissue labeling Olfactory Marker Protein/OMP with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat muscle. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)](https://www.abcam.co.jp/ps/products/183/ab183948/Images/ab183948-7-benefits-of-recombinant-antibodies.png)
Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab183948 は 1 報の論文で使用されています。
- Lee IC et al. Social Transmission and Buffering of Hippocampal Metaplasticity after Stress in Mice. J Neurosci 41:1317-1330 (2021). PubMed: 33310752