Anti-OGT / O-Linked N-Acetylglucosamine Transferase 抗体 (ab59135)
Key features and details
- Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
OGT / O-Linked N-Acetylglucosamine Transferase 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase -
由来種
Goat -
特異性
ab59135 is expected to recognize both reported isoforms of O-Linked N-Acetylglucosamine -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Cow, Dog, Pig, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide:
C-YEHPKDLKLSDGR
by a Cysteine residue linker, corresponding to amino acids 543-555 of Human O-Linked N-Acetylglucosamine -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59135の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
A 1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa. A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
組織特異性
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
ドメイン
The TPR repeat domain mediates recognition of protein substrates. -
翻訳後修飾
Ubiquitinated, leading to its proteasomal degradation. -
細胞内局在
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 532053 Cow
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 26295 Rat
- Entrez Gene: 337685 Zebrafish
- SwissProt: Q8CGY8 Mouse
- SwissProt: Q27HV0 Pig
- SwissProt: P56558 Rat
see all -
別名
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
画像
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135) at 0.05 µg/ml + Rat pancreas lysate in RIPA buffer at 35 µg
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Preliminary testing was unsuccessful on Mouse Brain for this particular batch.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab59135 は 3 報の論文で使用されています。
- Moura CS et al. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res 61:1290740 (2017). WB ; Rat . PubMed: 28326005
- Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . PubMed: 24465380
- Andrés-Bergós J et al. The increase in o-linked N-acetylglucosamine protein modification stimulates chondrogenic differentiation both in vitro and in vivo. J Biol Chem 287:33615-28 (2012). WB . PubMed: 22859309