Anti-OGDH 抗体 [EPR27181-78]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue lABeling OGDH with ab307369 at 1/2000 (0.232 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human cardiac muscle (PMID : 31682939). The section was incubated with ab307369 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue lABeling OGDH with ab307369 at 1/2000 (0.232 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human kidney. The section was incubated with ab307369 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized HeLa (human cervical adenocarcinoma epithelial cell) cells lABelling OGDH with ab307369 at 1/100 (4.64 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in HeLa cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab33985 Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 1ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling OGDH with ab307369 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling OGDH with ab307369 at 1/50 (9.28 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression : confocal image showing no staining on mouse spleen (PMID : 35540547). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307369 for 60 mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling OGDH with ab307369 at 1/50 (9.28 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307369 for 60 mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized NIH/3T3 (mouse embryonic fibroblast) cells lABelling OGDH with ab307369 at 1/100 (4.64 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in NIH/3T3 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling OGDH with ab307369 at 1/2000 dilution (0.232 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining on mouse kidney.
The section was incubated with ab307369 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling OGDH with ab307369 at 1/2000 dilution (0.232 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining on rat kidney.
The section was incubated with ab307369 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling OGDH with ab307369 at 1/2000 (0.232 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat cardiac muscle (PMID : 31682939). The section was incubated with ab307369 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling OGDH with ab307369 at 1/2000 (0.232 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse cardiac muscle (PMID : 31682939). The section was incubated with ab307369 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-OGDH antibody [EPR27181-78] (AB307369)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling OGDH with ab307369 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• WB

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Western blot - Anti-OGDH antibody [EPR27181-78] (AB307369)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3 seconds

All lanes:

Western blot - Anti-OGDH antibody [EPR27181-78] (ab307369) at 1/1000 dilution

Lane 1:

Human heart tissue lysate 20 μg

Lane 2:

Human kidney tissue lysate 20 μg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 116 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-OGDH antibody [EPR27181-78] (AB307369)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTThe identity of the lower MW band at approximately 40 kDa is unknown. Exposure time : 180 seconds

All lanes:

Western blot - Anti-OGDH antibody [EPR27181-78] (ab307369) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate 20 μg

Lane 2:

HeLa transfected with siRNA specifically targeti OGDH whole cell lysate 20 μg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 116 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-OGDH antibody [EPR27181-78] (AB307369)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 180 seconds

All lanes:

Western blot - Anti-OGDH antibody [EPR27181-78] (ab307369) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate 20 μg

Lane 2:

RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate 20 μg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 116 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-OGDH antibody [EPR27181-78] (AB307369)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLow expression tissue : spleen (PMID : 35540547). Exposure time : 15 seconds

All lanes:

Western blot - Anti-OGDH antibody [EPR27181-78] (ab307369) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate 20 μg

Lane 2:

Mouse kidney tissue lysate 20 μg

Lane 3:

Mouse spleen tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 116 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-OGDH antibody [EPR27181-78] (AB307369)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLow expression tissue : spleen (PMID : 35540547). Exposure time : 10 seconds

All lanes:

Western blot - Anti-OGDH antibody [EPR27181-78] (ab307369) at 1/1000 dilution

Lane 1:

Rat heart tissue lysate 20 μg

Lane 2:

Rat kidney tissue lysate 20 μg

Lane 3:

Rat spleen tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 116 kDa

false

Exposure time: 10s