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リコンビナントRabMAb

Anti-Oct6 抗体 [EPR24057-94] (ab259952)

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Western blot - Anti-Oct6 antibody [EPR24057-94] (ab259952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)
  • Immunohistochemistry (Frozen sections) - (ab259952)
  • Immunohistochemistry (Frozen sections) - (ab259952)
  • Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody [EPR24057-94] (ab259952)
  • Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody [EPR24057-94] (ab259952)
  • Flow Cytometry (Intracellular) - Anti-Oct6 antibody [EPR24057-94] (ab259952)
  • Flow Cytometry (Intracellular) - Anti-Oct6 antibody [EPR24057-94] (ab259952)
  • (ab259952)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24057-94] to Oct6
  • Suitable for: ICC/IF, WB, Flow Cyt (Intra), IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Oct6 antibody [EPR24057-94]
    Oct6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24057-94] to Oct6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, Flow Cyt (Intra), IHC-P, IHC-Frmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat P0 brain, Rat P7 hippocampus, Mouse P1 brain, F9, NCCIT lysates. IHC-P: Human skin, Mouse cerebrum and Rat skin tissues. IHC-Fr: Mouse E14.5 embryonic, Rat E14.5 embryonic tissues. ICC/IF: rat primary neuron, mouse primary neuron cellss. Flow Cyt (Intra): Mouse primary neuron, Rat primary neuron cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab259952の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50.
WB
1/1000. Predicted molecular weight: 45 kDa.
Flow Cyt (Intra)
1/50.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
特記事項
ICC/IF
1/50.
WB
1/1000. Predicted molecular weight: 45 kDa.
Flow Cyt (Intra)
1/50.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.

  • 組織特異性

    Expressed in embryonal stem cells and in the developing brain.

  • 配列類似性

    Belongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • class 3 antibody
    • Oct-6 antibody
    • OCT6 antibody
    • Octamer-binding protein 6 antibody
    • Octamer-binding transcription factor 6 antibody
    • OTF-6 antibody
    • OTF6 antibody
    • PO3F1_HUMAN antibody
    • POU class 3 homeobox 1 antibody
    • POU domain antibody
    • POU domain class 3 transcription factor 1 antibody
    • POU domain transcription factor SCIP antibody
    • pou3f1 antibody
    • SCIP antibody
    • transcription factor 1 antibody
    see all

画像

  • Western blot - Anti-Oct6 antibody [EPR24057-94] (ab259952)
    Western blot - Anti-Oct6 antibody [EPR24057-94] (ab259952)
    All lanes : Anti-Oct6 antibody [EPR24057-94] (ab259952) at 1/1000 dilution

    Lane 1 : Rat P0 brain tissue lysate
    Lane 2 : Rat P7 hippocampus tissue lysate
    Lane 3 : Rat lung tissue lysate
    Lane 4 : Mouse P1 brain tissue lysate
    Lane 5 : Mouse lung tissue lysate
    Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
    Lane 7 : NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 45 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration:  5% NFDM/TBST

    Negative control: lung (PMID: 1979677).

    Exposure time: 3 minutes

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on epithelial cells of human skin. The section was incubated with ab259952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse cerebrum. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)

    Immunohistochemical analysis of paraffin-embedded Rat skin tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on epithelial cells of rat skin. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in rat lung. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - (ab259952)
    Immunohistochemistry (Frozen sections) - (ab259952)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic tissue labeling Oct6 with ab259952 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on mouse E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - (ab259952)
    Immunohistochemistry (Frozen sections) - (ab259952)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat E14.5 embryonic tissue labeling Oct6 with ab259952 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on rat E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody [EPR24057-94] (ab259952)
    Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody [EPR24057-94] (ab259952)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Oct6 with ab259952 at 1/50 (10.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in rat primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody [EPR24057-94] (ab259952)
    Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody [EPR24057-94] (ab259952)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Oct6 with ab259952 at 1/50 (10.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Oct6 antibody [EPR24057-94] (ab259952)
    Flow Cytometry (Intracellular) - Anti-Oct6 antibody [EPR24057-94] (ab259952)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling Oct6 with ab259952 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Oct6 antibody [EPR24057-94] (ab259952)
    Flow Cytometry (Intracellular) - Anti-Oct6 antibody [EPR24057-94] (ab259952)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells cells labelling Oct6 with ab259952 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • (ab259952)
    (ab259952)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab259952 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab259952 は論文での使用が確認できていません。

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