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Anti-Oct4 抗体 (ab18976)

Reviews (16)Q&A (10)References (219)
Western blot - Anti-Oct4 antibody (ab18976)

    Key features and details

    • Rabbit polyclonal to Oct4
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Oct4 antibody [EPR17980] (ab200834)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Oct4 antibody
      Oct4 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Oct4

    • 由来種

      Rabbit

    • 特異性

      The antibody reacts with 45 kDa Oct4 (also designated Oct3A) and in a less extent 33 kDa Oct3B.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat
      交差が予測される動物種: Mouse, Human, Chimpanzee

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called 3576 Oct-4 Antibody. 3576-100 is the same size as the 100 µg size of ab18976.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab18976の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (5)
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.
    特記事項
    WB
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.

    ターゲット情報

    • 機能

      Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

    • 組織特異性

      Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

    • 配列類似性

      Belongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.

    • 発生段階

      Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.

    • ドメイン

      The POU-specific domain mediates interaction with PKM2.

    • 翻訳後修飾

      Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
      Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.

    • 細胞内局在

      Nucleus. Expressed in a diffuse and slightly punctuate pattern.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Octamer binding transcription factor 4 antibody
      • MGC22487 antibody
      • Oct 3 antibody
      • Oct 4 antibody
      • Oct-3 antibody
      • Oct-4 antibody
      • OCT3 antibody
      • Oct4 antibody
      • Octamer binding protein 3 antibody
      • Octamer binding protein 4 antibody
      • Octamer binding transcription factor 3 antibody
      • Octamer-binding protein 3 antibody
      • Octamer-binding protein 4 antibody
      • Octamer-binding transcription factor 3 antibody
      • OTF 3 antibody
      • OTF 4 antibody
      • OTF-3 antibody
      • OTF3 antibody
      • OTF4 antibody
      • PO5F1_HUMAN antibody
      • POU class 5 homeobox 1 antibody
      • POU domain class 5 transcription factor 1 antibody
      • POU domain transcription factor OCT4 antibody
      • POU domain, class 5, transcription factor 1 antibody
      • POU-type homeodomain-containing DNA-binding protein antibody
      • POU5F1 antibody
      see all

    画像

    • Western blot - Anti-Oct4 antibody (ab18976)
      Western blot - Anti-Oct4 antibody (ab18976)
      Anti-Oct4 antibody (ab18976) at 0.5 µg/ml + Lysates prepared from rat kidney tissue.

      Predicted band size: 45 kDa



      The antibody reacts with 45 kDa Oct4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B from human, mouse, and rat samples.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (219)

    ab18976 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab18976 は 219 報の論文で使用されています。

    • Kim J  et al. Transcriptional activation of endogenous Oct4 via the CRISPR/dCas9 activator ameliorates Hutchinson-Gilford progeria syndrome in mice. Aging Cell 22:e13825 (2023). PubMed: 36964992
    • Zheng K  et al. Chito-oligosaccharides and macrophages have synergistic effects on improving ovarian stem cells function by regulating inflammatory factors. J Ovarian Res 16:76 (2023). PubMed: 37060101
    • Leal Reis I  et al. Allogenic Synovia-Derived Mesenchymal Stem Cells for Treatment of Equine Tendinopathies and Desmopathies-Proof of Concept. Animals (Basel) 13:N/A (2023). PubMed: 37106875
    • Li X  et al. Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice. Front Immunol 14:1185921 (2023). PubMed: 37228612
    • Hamedi H  et al. Intravenous Transplantation of Adipose-Derived Mesenchymal Stem Cells Promoted The Production of Dopaminergic Neurons and Improved Spatial Memory in A Rat Model of Parkinson's Disease. Cell J 25:317-326 (2023). PubMed: 37300293
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 26 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Oct4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (H9c2 rat cardiomyocytes)
    Permeabilization
    Yes - 0.01% TritonX-100
    Specification
    H9c2 rat cardiomyocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 18 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Oct4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cardiomyocytes)
    Permeabilization
    Yes - 0.01% TritonX-100
    Specification
    Cardiomyocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 09 2020

    Western blot abreview for Anti-Oct4 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (C42B Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    C42B Prostate cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    投稿 Dec 25 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Oct4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (Swine adipose-derived mesenchymal stem cells induc)
    Permeabilization
    Yes - 0.1 % Triton X-100
    Specification
    Swine adipose-derived mesenchymal stem cells induc
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dominic Schomberg

    Verified customer

    投稿 Apr 24 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Oct4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (PC3 Prostate cancer cells)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    PC3 Prostate cancer cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    投稿 Mar 21 2019

    Immunocytochemistry abreview for Anti-Oct4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Neural stem cells)
    Permeabilization
    No
    Specification
    Neural stem cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Kyungjoo Seong

    Verified customer

    投稿 Oct 31 2016

    Flow Cytometry abreview for Anti-Oct4 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (H9 hESCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Fibroblasts (white)
    Specification
    H9 hESCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 24 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Oct4 antibody

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Fetal Brain)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    Fetal Brain
    Blocking step
    Dako protein block as blocking agent for 10 minute(s) · Concentration: .25% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Erica Sloma

    Verified customer

    投稿 Oct 09 2014

    Question

    Dear Tech Support Team,



    Please read below the details of customer's complaint:



    Antibody code: ab18976

    General Information:

    Antibody storage conditions (temperature/reconstitution etc) (-20).

    Description of the problem (high background, low signal, non-specific staining etc.) no staining in ImmunoFluorescence staning.

    Sample (Species/Tissue/Cell Type/Cell Line etc.) Human & mouse cells & tissue

    Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.) Cells: methanol, Human tissue: formaldehyde, Mouse tissue: bouin.

    Antigen retrieval (Enzymatic method, Heat mediated technique etc.) Urea

    Permeabilization step

    Blocking conditions (Buffer/time period, Blocking agent etc.) Fetal Calf Serum in PBSX1 for 40mins

    Primary Antibody (Diluent/Dilution/Incubation time, Wash step)1:100 and 1:200 in blocking buffer for over night at 4C and washes with PBSX1 for 3 times, 5mins each.

    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Fluorescent Anti Rabbit-1:1200 in blocking buffer for 40mins at room tempreture and washes in PBSX1 for 3 times, 10mins each.

    Detection method fluorescent microscope

    Positive and negative controls used (please specify) negative control: tissue/cells incubated with the secondary antibody without the first one.

    Positive control: tissues/cells stained with another antibody that works excellent (Sall-4 of abcam: ab

    Optimization attempts (problem solving):

    How many times have you tried the IHC? Our lab: since many years. Me: 2 years

    Have you run a "No Primary" control? Yes

    Do you obtain the same results every time? Yes

    What steps have you altered? All the steps are the same.



    The attached images:

    http://mail.zotal.co.il/exchange/Alla/Inbox/%D7%A6%D7%91%D7%99%D7%A2%D7%AA%20OCT%20%D7%91%D7%A8%D7%A7%D7%9E%D7%AA%20HUMAN.EML/1_multipart_xF8FF_3_Merged-30%2B31.jpg/C58EA28C-18C0-4a97-9AF2-036E93DDAFB3/Merged-30%2B31.jpg?attach=1 and http://mail.zotal.co.il/exchange/Alla/Inbox/%D7%A6%D7%91%D7%99%D7%A2%D7%AA%20OCT%20%D7%91%D7%A8%D7%A7%D7%9E%D7%AA%20HUMAN.EML/1_multipart_xF8FF_2_Merged-27%2B28.jpg/C58EA28C-18C0-4a97-9AF2-036E93DDAFB3/Merged-27%2B28.jpg?attach=1 - human sections, same dilutions, looks like the negative control



    All other attached images - mouse sections, 1/200 and 1/100, looks like the negative control



    Please advise.



    Thanks in advance.

    Have a nice week,

    Read More
    Answer

    Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody. The details your customer has kindly provided will enable us to investigate this case for your customer and this is also helpful in our records for monitoring of quality.
    Although the other antibody has worked well using this procedure, individual antibodies will often require optimization especially if the target is different. I would like to offer some suggestions to help optimise the results from ab18976.
    1.) I can recommend to add a permeabilization step to the protocol. Oct4 is in the nucleus. The following detergents can be used: Triton or NP-40 Use 0.1 to 0.2% in PBS for 10 min only. These will partially dissolve the nuclear membrane and are therefore very suitable for nuclear antigen staining.
    2.) Oct4 is expressed in developing brain. Highest levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. Since there is only a low level of expression in adult tissues, I suggest to run a positive control.
    I would also appreciate if your customer can confirm some details of the procedure.
    1.) What tissues and cells were used?
    2.) What does it urea mean as antigen retrieval step?
    3.) What cells are shown in the images?
    We are happy to offer this technical support. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.
    I hope this information is helpful, thank you and your customer for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

    Read More

    Question

    Dear Sir / Madam,
    One of the biggest customer in stem cell lab would like to have an
    antibody that is specific against Oct 4, either variant A or variant B
    to distinguish between them. In literature, oct 4 antibodies are not
    able to bind to specific variants.
    What do you recommend?
    Many Thanks and Best Regards,

    Read More
    Answer

    Thank you for your enquiry and your interest.

    I can confirm that two isoforms of Oct 4 have been identified, and the exact amino acid sequences (aa) can be found at this website: http://www.uniprot.org/uniprot/Q01860

    - Isoform A: Q01860-1 (1-360 aa)

    - Isoform B: Q01860-2 (1-265 aa)

    ab109183 - this antibody was raised against a peptide corresponding to the region between 265-360 aa, soit is very likely to recognize Isoform A.

    ab18976 - the immunogen selected was around region 1-14 aa, thus it would recognize potentially both Isofm A and B.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    1-10 of 26 Abreviews or Q&A

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